| 重组人表皮生长因子的构建与表达 |
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| 引用本文： | 李姗姗,朱晓美,鲍岩岩,朱大岭.重组人表皮生长因子的构建与表达[J].哈尔滨医科大学学报,2011,45(4):297-300. |
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| 作者姓名： | 李姗姗 朱晓美 鲍岩岩 朱大岭 |
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| 作者单位： | 1. 哈尔滨医科大学药学院生物制药教研室;省部共建教育部生物医药工程重点实验室,黑龙江哈尔滨150081
2. 省部共建教育部生物医药工程重点实验室,黑龙江哈尔滨,150081 |
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| 基金项目： | 黑龙江省教育厅科研项目(11531181) |
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| 摘 要： | 目的 应用原核表达系统研究提高重组表皮生长因子( rhEGF)产量的方法.方法 用RT-PCR方法克隆hEGF基因并插入表达载体pGEX-4T-1,获得重组的表达质粒pGEX-4T-1-hEGF转化大肠杆菌BL21并筛选得到工程菌株,以异丙基-β-D-硫代半乳糖苷诱导目的蛋白表达,亲和层析纯化rhEGF,MTT法测定活...
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| 关 键 词： | 人表皮生长因子 原核表达 生物活性 |
Construction and expression of recombinant human epidermal growth factor |
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| LI Shan-shan,ZHU Xiao-mei,BAO Yan-yan,ZHU Da-ling.Construction and expression of recombinant human epidermal growth factor[J].Journal of Harbin Medical University,2011,45(4):297-300. |
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| Authors: | LI Shan-shan ZHU Xiao-mei BAO Yan-yan ZHU Da-ling |
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| Institution: | LI Shan-shan1,2,ZHU Xiao-mei2,BAO Yan-yan2,ZHU Da-ling2(1.Department of Biopharmaceutical Sciences,College of Pharmacy,2.Biopharmaceutical Key Laboratory of Heilongjiang Province,Harbin Medical University,Harbin 150081,China) |
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| Abstract: | Objective To research the method for increasing the yield and activity of rhEGF by prokaryotic expression vector.Methods The human EGF gene was amplified by RT-PCR,and was inserted into prokaryotic expression vector pGEX-4T-1.After identified by restriction mapping and sequencing,the recombinant plasmid pGEX-4T-1-hEGF was transformed into E.coli BL21 cell.Recombinant was purified with affinity chromatography and its bioactivity was determined with MTT assay.Results The human EGF gene was amplified successfu... |
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| Keywords: | recombinant human epidermal growth factor prokaryotic expression biological activity |
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