| TNF-α在体外具有促进猪卵泡内膜细胞增殖的作用 |
| |
| 引用本文： | 洪岭,滕晓明,李昆明,龚瑶,张燕,杨芳,张博,童晓文.TNF-α在体外具有促进猪卵泡内膜细胞增殖的作用[J].华西医学,2010(1):46-48. |
| |
| 作者姓名： | 洪岭 滕晓明 李昆明 龚瑶 张燕 杨芳 张博 童晓文 |
| |
| 作者单位： | [1]同济大学附属第一妇婴保健院,上海200040 [2]同济大学附属同济医院妇产科,上海200065 |
| |
| 摘 要： | 目的：研究肿瘤坏死因子（TNF-α）对猪卵泡内膜细胞增殖情况的影响,探讨TNF-α在多囊卵巢综合征（P-COS）发病机制中的作用。方法：在体外培养的猪卵泡内膜细胞培养液中加入TNF-α（10、100和1000 pg/mL）,采用CFDA SE细胞增殖检测试剂盒和流式细胞仪观察TNF-α对卵泡内膜细胞增殖情况的影响。结果：72 h后TNF-α（100和1000 pg/mL）组卵泡内膜细胞平均荧光强度（MFI）明显低于对照组,有统计学差异。结论：TNF-α具有促进卵泡膜细胞增殖的作用,这个现象可能与PCOS患者发生卵巢白膜增厚、卵泡膜细胞增生病变有关。
|
| 关 键 词： | TNF-α PCOS CFDA SE 卵泡内膜细胞 |
TNF-alpha can Promote the Proliferation of Porcine Theca Interna Cells in Vitro |
| |
| Institution: | HONG Ling,TENG Xiao-ming,LI Kun-ming,et al.(The First Maternal and Infant Health Care Hospital,Tongji University,Shanghai 200040,China ) |
| |
| Abstract: | Objective： To study the effect of tumor necrosis factor-alpha（TNF-alpha） on the proliferation of theca interna cells（TI） and to explore the role of TNF-alpha in the polycystic ovary syndrome pathogenesis.Methods： Different concentrations of TNF-αlpha（10 pg/mL,100 pg/mL,1000 pg/mL） were treated with TI.Proliferation of TI were detected by CFDA SE kit and Flow Cytometry.Results： After 72 h,The mean fluorescence intensity（MFI） in TI with TNF-alpha 100 pg/mL and 1000 pg/mL were lower obviously than controls（P〈0.05）.Conclusion： TNF-αlpha can promote the proliferation of theca interna cells,which may explain the phenomenon： PCOS usually accompanies thick ovarian tunica albuginea and proliferative theca cells. |
| |
| Keywords: | TNF-α PCOS CFDA SE theca interna cells |
| 本文献已被 维普 等数据库收录！ |
|
