斑蝥素通过MAPK信号传导途径对A549细胞增殖抑制作用的研究

目的 :研究斑蝥素对人肺癌A5 49细胞的增殖抑制作用和有丝分裂原激活蛋白激酶 (mi togenactivatedproteinkinase ,MAPK)表达的影响。方法 :采用MTT法检测斑蝥素对A5 49细胞的增殖抑制作用 ;流式细胞仪分析A5 49细胞周期及斑蝥素对细胞周期的影响 ;蛋白印迹法检测斑蝥素作用后ERK1、ERK2 、phos ERK1、phos ERK2 表达的改变。结果 :斑蝥素对A5 49细胞有增殖抑制作用 ;A5 49细胞周期出现明显的G2 /M期阻滞 ,t检验P <0 0 5 ;ERK1、ERK2 、phos ERK1、phos ERK2表达下降。结论 :MAPK信号传导途径参与了斑蝥素对人肺癌A5 49细胞的增殖抑制作用。

Study of proliferation inhibition effect of cantharidin on human lung carcinoma A549 cell through MAPK signal transduction pathway

OBJECTIVE: To investigate the effect of cantharidin on the proliferation of human lung carcinoma A549 cell and mitogen activated protein kinase (MAPK). METHODS: MTT assay was used to determine the proliferation inhibition by cantharidin on A549 cell. FACScan and Western blotting were applied to detect the change of cell cycle and expression levels of ERK 1, ERK 2, phos-ERK 1 and phos-ERK 2. RESULTS:Cantharidin inhibited the proliferation of A549 cell dramaticly and retarded A549 cell cycle at G 2/M phase. The expression levels of ERK 1, ERK 2, phos-ERK 1, phos-ERK 2 reduced.CONCLUSION:MAPK signal transduction pathway participates the proliferation inhibition of cantharidin on A549 cell.