Immunohistochemical localization of a new thiamine diphosphate-binding protein in the rat nervous system |
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| Authors: | H Yoshioka K Nishino T Miyake G Ohshio T Kimura Y Hamashima |
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| Affiliation: | 1. Department of Pathology, Faculty of Medicine, Kyoto University, Kyoto Japan;2. Department of Hygiene, Faculty of Medicine, Kyoto University, Kyoto Japan;3. Department of Geriatric Medicine, Faculty of Medicine, Kyoto University, Kyoto Japan;4. Department of Neurology, Faculty of Medicine, Kyoto University, Kyoto Japan;1. College of Life Science, Shandong Agricultural University, Shandong, PR China;2. Shandong Agricultural Technology Extending Station, Ji''nan, Shandong, 250100, PR China;3. The Chilingzhi Development Co., Ltd., of Taian, Shandong, PR China;1. Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran-Iran (Center of Excellent of Ecosystem and Ultrastructural changes of Helminthes), Iran;2. Department of Medical Entomology & Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran;1. School of Public Health, Key Laboratory of Public Health Safety, NHC Key Laboratory of Health Technology Assessment, Fudan University, Shanghai, 200032, China;2. Deqing County Center for Disease Control and Prevention, Deqing, 313299, China;3. School of Epidemiology and Public Health, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, K1G 5Z3, Canada;1. Department of Physiology, Shandong University School of Medicine, 44#, Wenhua Xi Road, Jinan, Shandong 250012, PR China;2. Department of Neurosurgery, Qilu Hospital of Shandong University and Brain Science Research Institute, Shandong University, 107#, Wenhua Xi Road, Jinan, Shandong 250012, PR China;3. Department of Medical Psychology Shandong University School of Medicine, 44#, Wenhua Xi Road, Jinan, Shandong 250012, PR China;1. Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI 48109, United States;2. Division of Metabolism, Endocrinology, and Diabetes, Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, United States;3. Department of Pharmacology, University of Michigan, Ann Arbor, MI 48109, United States;1. Department of Physiology, Shandong University School of Medicine, 44#, Wenhua Xi Road, Jinan, Shandong, 250012, PR China;2. Department of Medical Psychology, Shandong University School of Medicine, 44#, Wenhua Xi Road, Jinan, Shandong 250012, PR China;3. Department of Ultrasound, Qilu hospital of Shandong University, 107#, Wenhua Xi Road, Jinan, Shandong 250012, PR China |
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| Abstract: | ![]()
The neurophysiological roles of thiamine-binding proteins have lately attracted considerable attention. We purified a new thiamine diphosphate-binding protein (ThDP-BP) prepared from rat livers and produced an antiserum against it. We examined the immunohistochemical distribution of ThDP-BP in the rat nervous system by the avidin-biotin complex technique. Immunoreactivity for ThDP-BP was found in several groups of neurons, and other neuronal tissues including glial cells, ependymal cells and Schwann cells. In neurons, the cytoplasm and processes were stained, but the nucleus was scarcely stained. In glial cells, the nucleus was stained, but the cytoplasm was not stained. |
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