Animal model of vascular dementia and its cholinergic mechanism |
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| Authors: | FAN Wen-hui LI Lu-si LIU Zhi-rong ZHU Hong-yan CHEN Kang-ning |
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| Affiliation: | Department of Neurology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China Department of Neurology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China Department of Neurology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China Department of Neurology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China Department of Neurology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China |
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| Abstract: | Objective: To establish a model of vascular dementia (VD) in aging rats and study primarily the cholinergic mechanism of hypomnesia. Methods: Chronic hypoperfusion of cerebral blood flow (CBF) in the forebrain was performed in aging rats with permanent bilateral common carotid arteries occlusion (PBCCAO). Then the rats were tested with a computerized shuttle-training case. The changes of cerebrovascular system were observed with digital subtraction angiography (DSA). The brain tissues were studied with immunohistochemical method with cholinergic acetyltransferase (ChAT) as a marker. Results: The cognitive function of rats was obviously reduced in 2 months after chronic cerebral hypoperfusion and became worse 2 months later, showing a more marked decrease of ChAT positive neurons and fibers in CA1 of the hippocampus as compared with the rats of the control, which had a significant positive correlation with memory ability. Conclusion: This rat model is successfully established to imitate human VD induced with chronic cerebral hypoperfusion. The mechanism of the hypomnesia of VD might be the impairment of cholinergic neurons in frontal cortex and hippocampus. |
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| Keywords: | vascular dementia memory disorders animal model cholinergic acetyltransferase |
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