乌司他丁对脓毒症急性肺损伤大鼠热休克蛋白70表达的影响

目的研究热休克蛋白(HSP)70在脓毒症急性肺损伤(ALI)大鼠中的表达变化及应用乌司他丁(UTI)进行干预,并探讨作用机制。方法 39只Wistar大鼠分为假手术(sham)组(9只)、ALI组(15只)和UTI组(15只)。大鼠盲肠结扎穿孔术后6、12、24 h分别处死大鼠。检测血气分析、肺组织湿/干重比(W/D),光镜下观察肺组织形态学变化,应用实时荧光定量-聚合酶链反应(realtime-PCR)测定肺组织HSP70 mRNA的表达。结果与ALI组比较,UTI组PaO2显著改善(P<0.05),肺W/D比值降低(P<0.05),苏木精-伊红染色显示肺组织损伤减轻。各时间点肺组织HSP70 mRNA表达明显上升(P<0.05)。结论 HSP70在脓毒症ALI大鼠中的表达上调,UTI可能通过上调HSP70的表达改善脓毒症肺组织的损伤。

Effect of Ulinastatin on heat shock protein 70 expression in rats with septic acute lung injury

Objective To study heat shock protein 70（HSP70） expression and investigate the possible mechanisms of Ulinastatin therapy in rats with septic acute lung injury（ALI）.Methods Thirty-nine Wistar rats were randomly divided into sham group（n = 12）,ALI group（n = 15） and Ulinastatin（UTI） group（n = 15）.And rats were sacrificed at 6,12 and 24 hours after cecal ligation and puncture operation.Arterial blood gas analysis,lung tissue wet/dry weight ratio,lung histopathologica1 changes and pulmonary expression of HSP70 mRNA assessed by realtime-PCR were observed.Results Compared with ALI group,PaO2 in UTI group was significantly increased whereas the ratio of wet/dry weight was decreased（P 0.05）.The grading of pathologic damage of lung tissue was attenuated greatly.Pulmonary expression of HSP70 mRNA were increased in UTI group compared to those in ALI group at all time points（P 0.05）.Conclusion Effects of Ulinastatinon on the improvement of septic acute lung injury may be attributed to the up-regulation of lung HSP70 expressions.