清脑宣窍滴丸对大鼠急性脑缺血再灌注损伤保护作用的研究

目的 :研究清脑宣窍滴丸对急性脑缺血损伤的保护作用。 方法 :ig给予清脑宣窍滴丸(180,90,45 mg·kg^-1剂量),每天1次,连续3 d,采用线栓法致大鼠大脑中动脉缺血再灌注(MCAO)模型,缺血2 h再灌注22 h,评分法评价神经损伤行为症状异常;放射免疫法检测血清中神经元特异性烯醇化酶(NSE)的活性;HE染色法观察脑组织病理形态的变化。 结果 :各给药组可不同程度改善损伤模型大鼠6 h和22 h的神经缺损症状,清脑宣窍滴丸180 mg ·kg^-1组与模型组比较有显著差异(P<0.05);清脑宣窍滴丸 45,90,180 mg ·kg^-1组可降低模型大鼠NSE活性;病理结果显示,清脑宣巧滴丸组正常神经细胞数量明显增多,变性坏死组织范围缩小、程度减轻。 结论 :清脑宣窍滴丸对脑缺血有保护作用。

Protective Effect of Qingnao Xuanqiao Dropping Pill on Experimental Ischemic Injury in Rats

Objective: To study the protective effect of Qingnao Xuanqiao Dropping Pill on acute cerebral ischemia in rats. Method: Intragastric gavage of Qingnao Xuanqiao Dropping Pill (high, medium and low dose), once a day for three days. The last day animal model of middle cerebral artery occlusion (MCAO) induced by suture-occluded method was adopted. Scoring method of ischemia for 2 h and reperfusion for 22 h was used to evaluate the abnormal behaviour by nerve injury. Radioimmunoassay was used to evaluate NSE activity in serum. HE staining was employed to observe pathological morphology in brain tissue. Result: Each administrated group could improve the nerve defect symptoms of model rats at 6 h and 22 h to some extent. Compared with model group, 180 mg ·kg^-1 group of Qingnao Xuanqiao Dropping Pill was significantly different (P<0.05); 45, 90, 180 mg ·kg^-1 group of Qingnao Xuanqiao Dropping Pill could reduce NSE activity of the model rats. Pathological results showed the number of normal nerve cells in Qingnao Xuanqiao Dropping Pill group was increased significantly, the scope and the degree of necrotic tissue was reduced. Conclusion: Qingnao Xuanqiao Dropping Pill has a protective effect on cerebral ischemia.