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| 紫外分光光度法测定川射干总黄酮的含量 | |
| 引用本文： | 张潇,李蓉,唐斌. 紫外分光光度法测定川射干总黄酮的含量[J]. 四川生理科学杂志, 2010, 32(2): 78-79 |
| 作者姓名： | 张潇 李蓉 唐斌 |
| 作者单位： | 1. 泸州医学院化学教研室,四川,泸州,646000 2. 泸州医学院药理毒理学研究室,四川,泸州,646000 |
| 摘 要： | 目的：建立川射干总黄酮含量的测定方法。方法：采用紫外分光光度法测定川射干药材中的射干苷含量，测定波长为266nm。结果：射干苷在0．002-0．010mg·mL^-1范围内与吸光度呈良好的线性关系，r=0．9991，回收率为97．45％，RSD为0．65％，川射干总黄酮的平均含量为4．17％。结论：该方法操作简便、灵敏、准确、具有良好的重复性和回收率，可作为川射干总黄酮含量测定的方法。 |
| 关 键 词： | 紫外分光光度法 川射干 总黄酮 含量 |
Determination of total flavonoids in Chuanshegan by UV spectrophotometry |
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| Zhang Xiao,Li Rong,Tang Bin. Determination of total flavonoids in Chuanshegan by UV spectrophotometry[J]. Sichuan Journal of Physiological Sciences, 2010, 32(2): 78-79 | |
| Authors: | Zhang Xiao Li Rong Tang Bin |
| Affiliation: | 1. Department of Chemistry of Luzhou Medical College; 2. Laboratory of Pharmacology and Toxicology of Luzhou Medical College, Luzhou Siehuan 646000) |
| Abstract: | Objective： To establish a method for the determination of total flavonoids in Chuanshegan. Methods. The contents of total flavonoids in Chuanshegan were determined by UV spectrophotometry with a detection wavelength at 266nm. Results. Good linear relationship with absorbability occurred when the concentration range of tectoridin was 0. 002-0. 010 mg· mL^-1 （r=0. 9991） and its recovery was 97. 45%（RSD=0. 65%）. The sample contained total flavonoids 4. 17%. Conclusions.The method is simple, sensitive, accurate and with good repeatability and recovery. It can be used to determine the content of total flavonoids in Chuanshegan. |
| Keywords: | UV spectrophotometry Chuanshegan Total flavonoids Content |
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