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| cbfa1反义核酸对体外培养小鼠牙胚发育的影响 | |
| 引用本文： | 李锋,吴补领,余擎. cbfa1反义核酸对体外培养小鼠牙胚发育的影响[J]. 牙体牙髓牙周病学杂志, 2004, 14(12): 663-666 |
| 作者姓名： | 李锋 吴补领 余擎 |
| 作者单位： | 第四军医大学口腔医学院,陕西,西安,710032 |
| 基金项目： | 国家自然科学基金资助项目(30271418) |
| 摘 要： | 目的：采用反义核酸技术，用特异的、与cbfa1 mRNA互补的AS—ODN，抑制cbfa1在体外培养牙胚的翻译，观察它对牙齿发育及矿化的影响，探讨其在牙齿发育中的作用。方法：设计合成cbfa1反义核酸，应用所建立的牙胚体外培养体系，用光镜和电镜观察小鼠牙胚被cbfa1反义核酸阻断表达后发育的情况。结果：在缺乏cbfa1情况下，与对照组相比，实验组牙胚在体外虽然可以继续生长发育至钟状晚期，但形成的基质较薄，且基质中胶原纤维排列稀疏，粗细不等，方向杂乱。此外，成釉细胞与成牙本质细胞的发育均显幼稚。结论：cbfa1可能不仅仅参与了牙齿的矿化，还在成牙本质细胞和成釉细胞的分化中起重要的作用。 |
| 关 键 词： | 核心结合因子a1 牙齿发育 牙胚培养 反义核酸 |
| 文章编号： | 1005-2593(2004)12-0663-04 |
| 修稿时间： | 2004-09-09 |
Study on the effect of cbfa1 AS -ODN on tooth development in vitro |
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| LI Feng,WU Bu-ling,YU Qing. Study on the effect of cbfa1 AS -ODN on tooth development in vitro[J]. Chinese Journal of Conservative Dentistry, 2004, 14(12): 663-666 | |
| Authors: | LI Feng WU Bu-ling YU Qing |
| Abstract: | AIM:To discuss the effect of cbfa1 on tooth development and mineraliztion by organ culture in vitro and antisense technology.METHODS:We designed and prefabricated AS-ODN.Some explants were cultured in the presence of antisense oligodeoxynucleotide targeted to cbfa1 mRNA.On the terminal date,the cultured explants were all examined by transmission electron microscope.RESULTS:Ultrastructure analyses showed that on average the matrix of AS-ODN treated group was thinner than that in the control group.Furthermore,the collagen fibers in extracellular matrix were found disorganized.The development of odontoblasts and ameloblasts was less mature than those in the control group.CONCLUSION:cbfa1 might participated in the progress of tooth development and mineralization.It could also induce the differentiation of odontoblasts and ameloblasts. [ |
| Keywords: | cbfa1 tooth development tooth germ organ culture antisense oligodeoxynucleotide |
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