腹腔注射舒芬太尼对SD大鼠气道反应性的影响

目的:观察腹腔注射舒芬太尼对SD大鼠气道反应性的影响。方法:将32只雄性SD大鼠按随机数字表法分为4组,正常对照为A组( n＝8,腹腔注射生理盐水,3 ml);实验组,B1组( n＝8,腹腔注射舒芬太尼60 μg/kg,3 ml)、B2组( n＝8,腹腔注射舒芬太尼90 μg/kg,...

Effects of intraperitoneal injection of sufentanil on airway responsiveness in sprague dawley rats

Objective To observe the effect of intraperitoneal injection of sufentanil on airway responsiveness in SD Rats.Methods Totally 32 male SD rats were randomly divided into 4 groups according to the random number table:control group A(n=8,intraperitoneal injection of normal saline,3 ml);group B(group B1,n=8,intraperitoneal injection of sufentanil 60μg/kg,3 ml;group B2 n=8,intraperitoneal injection of sufentanil 90μg/kg,3 ml;and group B3 n=8,intraperitoneal injection of sufentanil 120μg/kg,3 ml).On the first day,airway resistance and airway reactivity were measured by non-invasive airway resistance apparatus in four groups of rats as a reference baseline.On the 15th day,four groups of rats were given intraperitoneal injection intervention measures,and the airway resistance(sRaw,cmH2O·s)was measured 1,2,3,5,10 min after intraperitoneal injection.On the 30th day,four groups of rats were given intraperitoneal injection intervention measures according to the requirements of the group,and airway reactivity was measured 3 min later.T test and Fisher exact probability method were used to analyze the experimental data.Results On 1st day,compared with control group A,there was no significant difference in baseline airway resistance(3.27±0.74 vs.3.35±0.65,3.30±0.81 vs.3.35±0.65,3.41±0.68 vs.3.35±0.65)in groups B1,B2 and B3 before intraperitoneal injection(t=0.230,P>0.05,t=0.136,P>0.05,t=0.180,P>0.05).On the 15th day of the experiment,compared with the airway resistance of control group A at 1,2,3,5 and 10 min after intervention,there were no significant difference in the airway resistance of B1 group at 1 min and 2 min(4.15±1.27 vs.3.95±0.89,4.74±1.05 vs.3.78±0.85,t=0.365,P>0.05,t=2.010,P>0.05),there was statistically significant difference in airway resistance at 3 min and 5 min(4.92±1.33 vs.3.55±0.69,4.89±1.43 vs.3.47±0.71,t=2.586,P<0.05,t=2.516,P<0.05),and there was no statistically significant difference in airway resistance at 10 min(3.86±1.28 vs.3.41±0.69,t=0.875,P>0.05).There was no significant difference in 1 min airway resistance of B2 group(4.77±1.15 vs.3.95±0.89,t=1.595,P>0.05),and there was significant difference in airway resistance at 2,3 and 5 min(6.01±1.47 vs.3.78±0.85,6.95±1.68 vs.3.55±0.69,5.75±1.52 vs.3.47±0.71,t=3.715,P<0.05,t=5.295,P<0.05,t=3.844,P<0.05).There was no significant difference in airway resistance at 10 min(3.95±1.18 vs.3.41±0.69,t=1.117,P>0.05).There were significant differences in airway resistance of B3 group at 1,2,3 and 5 min(5.14±1.25 vs.3.95±0.89,7.91±1.35 vs.3.78±0.85,8.15±1.48 vs.3.55±0.69,7.69±1.23 vs.3.47±0.71,t=2.194,P<0.05,t=7.322,P<0.05,t=7.968,P<0.05,t=8.404,P<0.05).There was no significant difference in airway resistance at 10 min(4.16±1.08 vs.3.41±0.69,t=1.655,P>0.05);On the 30th day,the positive rate of airway reactivity in group B1 was 37.5%with no significant difference(P>0.05),75%in group B2(P<0.05),and 87.5%in group B3(P<0.05).Conclusion The effects of intraperitoneal injection of sufentanil on airway resistance and responsiveness in SD rats were dose dependent,and the effects of sufentanil on airway respiratory system were significant.But this kind of change increases gradually in a short time and reaches the peak,then gradually weakens.