乙苯对HEI-OC1细胞增殖及氧化应激水平的影响

目的研究乙苯染毒后耳蜗毛细胞株(HEI-OC1)细胞增殖及氧化指标的变化。方法于2019年7至12月,设置乙苯浓度分别为0、30、60、90、300、600、900 μmol/L和3、6、9、10 mmol/L,共11个浓度组,用CCK-8法测定乙苯染毒24 h后HEI-OC1细胞增殖活性;以0、1、2、4、8、16、32、64 mmol/L乙苯处理细胞24 h,计算乙苯的半数抑制浓度。用0、6、9和12 mmol/L乙苯染毒HEI-OC1细胞24 h后,测定细胞内丙二醛(MDA)含量、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活力。结果与0 mmol/L浓度组比较,6、9、12 mmol/L浓度HEI-OC1细胞存活率明显降低(P<0.01)。乙苯对HEI-OC1细胞的半数抑制浓度为12.86 mmol/L(R2=99.05)。不同浓度组(0、6、9、12 mmol/L)乙苯处理HEI-OC1细胞24 h,MDA含量、SOD和GSH-Px活力差异均有统计学意义(F=65.11、6.48、22.85,P<0.05);与0 mmol/L浓度组比较,9、12 mmol/L浓度组HEI-OC1细胞MDA含量明显升高,12 mmol/L浓度组SOD活力明显降低,6、12 mmol/L浓度组GSH-Px活力明显降低,差异均有统计学意义(P<0.05)。结论乙苯可以抑制HEI-OC1细胞增殖,引起细胞氧化损伤。

The effects of ethylbenzene on HEI-OC1 cells proliferation and oxidative stress level

Objective To study the changes of proliferation and oxidation indexes of Cochlear hair cell line(HEI-OC1 cells)exposed to ethylbenzene.Methods From July to December 2019,11 groups with ethylbenzene concentrations of 0,30,60,90,300,600,900μmol/L and 3,6,9,10 mmol/L,were used to determine the proliferation activity of HEI-OC1 cells exposed to ethylbenzene for 24 hours,and the cells were treated with 0,1,2,4,8,16,32,64 mmol/L ethylbenzene for 24 hours,then the 50%inhibitory concentration of ethylbenzene was calculated.After HEI-OC1 cells were exposed to 0,6,9 and 12 mmol/L ethylbenzene for 24 hours,the malondialdehyde(MDA)content,superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)activities were measured.Results Compared with 0 mmol/L concentration group,the survival rate of HEI-OC1 cells at 6,9,12 mmol/L concentration was significantly decreased(P<0.01).The 50%inhibitory concentration of ethylbenzene on HEI-OC1 cells was 12.86 mmol/L(R2=99.05).There were significant differences in SOD and GSH-Px activity in HEI-OC1 cells treated with ethylbenzene at different concentrations(0,6,9,12 mmol/L)for 24 hours(F=65.11,6.48,22.85,P<0.05).Compared with 0 mmol/L concentration group,the MDA content of HEI-OC1 cells was significantly increased in 9 and 12 mmol/L concentration groups,the SOD activity was significantly decreased in 12 mmol/L concentration group,and the GSH-Px activity was significantly decreased in 6 and 12 mmol/L concentration groups.Conclusion Ethylbenzene can inhibit the proliferation of HEI-OC1 cells and cause oxidative damage.