| HPLC法测定羌黄颈康丸中葛根素的含量 |
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| 引用本文： | 王静. HPLC法测定羌黄颈康丸中葛根素的含量[J]. 北方药学, 2014, 0(11): 8-10 |
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| 作者姓名： | 王静 |
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| 作者单位： | 漯河市食品药品检验所 漯河 462000 |
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| 摘 要： | 目的：建立HPLC法测定羌黄颈康丸中葛根素的含量。方法：以十八烷基硅烷键合硅胶为填充剂,流动相为甲醇∶水（21∶79）,流速为1.0ml/min,检测波长为250nm,柱温为35℃。结果：葛根素在上述色谱条件下,与其他组分有较好的分离度,葛根素线性范围为0.0505~0.303μg,r=0.9996,平均回收率为99.1%,RSD=0.5%（n=6）。结论：所建方法操作简单,重现性好,结果准确,可作为羌黄颈康丸中葛根素的含量测定方法。
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| 关 键 词： | 羌黄颈康丸 葛根素 含量测定 高效液相色谱法 |
Determination of Puerarin in Qianghuangjingkang pills by HPLC |
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| Wang Jing. Determination of Puerarin in Qianghuangjingkang pills by HPLC[J]. BEIFANG YAOXUE, 2014, 0(11): 8-10 |
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| Authors: | Wang Jing |
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| Affiliation: | Wang Jing;Luohe Institute for Food and Drug Control; |
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| Abstract: | Objective：To establish a HPLC method for determining the content of Puerarin in Qianghuangjingkang pills.Methods：C18 column was adopted with methanol-water（21∶79） as the mobile phase at 1.0ml/min.The detection wave length was at 250nm and column temperature was 35℃.Results：There was a good resolution between Puerarin and other components in this chromatographic condition.The linear range of Puerarin was 0.0505~0.303μg,r=0.9996.The average recovery rate was 99.1%,RSD=0.5%（n=6）.Conclusion：The method is simple, accurate sensitive and suitable for the determination of Puerarin content in Qianghuangjingkang pills. |
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| Keywords: | Qianghuangjingkang pills Puerarin Determination of content HPLC |
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