| ATM参与辐射损伤细胞学反应的研究 |
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| 引用本文： | 孟祥兵,宋宜,毛建平,巩波,董燕,刘斌,孙志贤.ATM参与辐射损伤细胞学反应的研究[J].中华放射医学与防护杂志,2000,20(3):149-152. |
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| 作者姓名： | 孟祥兵 宋宜 毛建平 巩波 董燕 刘斌 孙志贤 |
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| 作者单位： | 北京放射医学研究所生物化学室，北京放射医学研究所生物化学室，北京放射医学研究所生物化学室，兰州大学生物系，北京放射医学研究所生物化学室，北京放射医学研究所生物化学室，北京放射医学研究所生物化学室 |
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| 基金项目： | 国家自然科学基金资助项目! ( 3 970 0 0 3 8)，全军医药
卫生九五重点基金资助项目! ( 960 50 0 7) |
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| 摘 要： | 目的 克隆ＡＴＭ全长ｃＤＮＡ及含特异功能域的ｃＤＮＡ片段，寻找与ＡＴＭ相互作用的蛋白，分析ＡＴＭ在ＤＮＡ损伤名的分子机理。方法 利用长片段ＰＣ白话 增法，从人外周血来源的ｃＤＮＡ库中扩增ＡＴＭｃＤＮＡ；利用酵母双杂交系统筛选人外周血来源的ｃＤＮＡ库中与ＡＴＭ相互作用的蛋白。结果 经重叠ＰＣＲ扩增到ＡＴＭ全长ｃＤＮＡ，并筛选到数条与ＡＴＭＰ１３Ｋ激酶区相互作用的ｃＤＮＡ，并对其序列进行分析。结果 Ａ
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| 关 键 词： | 辐射损伤 ATM 细胞学反应 酵母双奈 |
| 收稿时间： | 1999/12/8 0:00:00 |
| 修稿时间： | : |
Participation of ATM in cellular response to DNA damage induced by ionizing radiation |
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| MENG Xiangbing,SONG Yi,MAO Jianping.Participation of ATM in cellular response to DNA damage induced by ionizing radiation[J].Chinese Journal of Radiological Medicine and Protection,2000,20(3):149-152. |
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| Authors: | MENG Xiangbing SONG Yi MAO Jianping |
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| Institution: | MENG Xiangbing,SONG Yi,MAO Jianping,et al.\ Department of Biochemistry,Institute of Radiation Medicine,Beijing 100850,China\, |
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| Abstract: | Objective\ To clone ATM full length cDNA and cDNA fragments containing some functional domains and to identify proteins that interact with ATM and mediate DNA damage signal transduction in cellular response to DNA damage. Methods\ ATM cDNA was amplified from Marthom TM \|Ready cDNA kit of human leukocytes by LD\|PCR.ATM\|interacting proteins were screened by yeast two hybrid system. Results\ ATM full\|length cDNA and cDNA fragments containing PI3K kinase domain,leucine zipper and proline rich region were amplified from human cDNAs.Several candidate clones that interacted with ATM PI3K domain were identified. Conclusion\ ATM mediates DNA damage signal transduction by interacting with many proteins. |
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| Keywords: | ATM \ P13K \ Domain \ LD\|PCR \ Yeast two\|hybrid system |
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