银屑病患者角质形成细胞VEGF表达的研究

目的　研究银屑病发病与血管内皮生长因子 (VEGF)的关系 ,探讨银屑病可能的发病机制。方法　①用免疫组化法检测银屑病患者皮损和非皮损处皮肤、正常健康人皮肤及体外培养的银屑病患者和正常人角质形成细胞(KC)VEGF的表达 ;②用双抗体夹心ELISA法检测银屑病患者及正常人KC培养上清液中VEGF含量。结果　①银屑病皮损处VEGF表达明显高于非皮损处和正常人皮肤 (P均 <0 .0 0 1) ,非皮损处与正常人皮肤VEGF表达也有显著性差异 (P <0 .0 5 ) ;②体外培养的银屑病皮损处和非皮损处KCVEGF表达明显高于正常人 (P均 <0 .0 0 1) ;银屑病皮损处KC与非皮损处KC相比VEGF表达也有显著性差异 (P <0 .0 5 ) )。结论　VEGF可能参与银屑病的发病。

The Study of Vascular Endothelial Growth Factor Expression in Psoriatic Keratinocytes

Objective To investigate the relationship between VEGF and the onset of psoriasis and to explore the possible pathogenesis of psoriasis.Methods ①Immunohistochemical techniques were empolyed to study the expression of VEGF in the involved and uninvolved skin of patients with chronic plaque psoriasis,and the skin of normal control subjects;The expression of VEGF was studied with immunohistochemical technique on the cultured psoriatic keratinocytes and normal control subjects; ②Levels of VEGF in cell-conditioned media were measured using quantitative sandwich enzyme-linked monoclonal immunosorbent assay.Results ①There was a significantly stronger expression of VEGF of the keratinocytes in the lesional skin than that in nonlesional skin and the normal control subjects( P <0.001, respectively),and significant difference was found of the non-lesional skin as compared with normal control subject( P <0.05);②Cultured psoriatic keratinocytes (from lesional or non-lesional skin) expressed greater VEGF than that from normal control keratinocytes( P <0.001,respectively),while cultured lesional psoriatic keratinocytes expressed more VEGF than that from non-lesional psoriatic keratinocytes( P <0.05).Conclusion VEGF may participate the pathogenesis of psoriasis.