| 人趋化因子受体CCR4的克隆与表达 |
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| 引用本文： | 王应,韩文玲,张颖妹,杨田,马大龙.人趋化因子受体CCR4的克隆与表达[J].中国免疫学杂志,2001,17(3):123-124,129. |
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| 作者姓名： | 王应 韩文玲 张颖妹 杨田 马大龙 |
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| 作者单位： | 北京大学医学部免疫系分子免疫室，北京 100083 |
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| 摘 要： | 目的：建立pcDI-CCR4稳定转染并具有CCR4功能性表达的细胞株，为深入研究CCR4配体提供基础。方法：利用RT－PCR技术进行人CCR4的cDNA克隆化。通过基因转染技术获得稳定转染细胞株，利用趋骅实验、钙流实验证实稳定转染细胞能有效表达CCR4并具有生物功能。结果：成功地将CCR4 cDNA克隆于真核表达质粒pcDI，转染HEK293细胞获得稳定表达CCR4的HEK293细胞，可被RANTES诱导产生趋化反应和钙内流反应。结论：建立的pcDI－CCR4稳定转染细胞株能有效表达CCR4并具有生物功能。
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| 关 键 词： | CCR4转染 HEK293细胞 人趋化因子受体 克隆 |
| 文章编号： | 1000-484X(2001)03-0123-03 |
Molecular cloning and
functional expression of human chemokine receptor CCR4 |
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| WANG Ying,HAN Wen Ling,ZHANG Ying Mei et al.Molecular cloning and
functional expression of human chemokine receptor CCR4[J].Chinese Journal of Immunology,2001,17(3):123-124,129. |
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| Authors: | WANG Ying HAN Wen Ling ZHANG Ying Mei |
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| Institution: | WANG Ying,HAN Wen Ling,ZHANG Ying Mei et al.Department of Immunology,Peking University Health Science Center,Beijing 100083 |
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| Abstract: | Objective:To constructed a functional cell line stably transfected with human CCR4 cDNA and to study the interaction of CCR4 and its ligand Methods:RT PCR, cDNA cloning and transfecting were used for the constructing of the functional cell line stably expressing human CCR4 The chemotaxis and calcium mobilization assay were used for examining bioactivities of the cells stably transfected with CCR4 Results:CCR4 cDNA was successfully cloned into the eukaryotic vector pcDI and pcDI CCR4 was stably transfected into HEK293 cells, which can be induced a chemotactic responses and the calcium mobilization by RANTES Conclusion:We have constructed a functional cell line stably expressing human CCR4 |
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| Keywords: | CCR4 transfection HEK293 cell |
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