| PAI-1对鼻咽癌侵袭和转移的影响 |
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| 引用本文： | 潘国庆,金树珍,付红梅,边莉,唐慰萍. PAI-1对鼻咽癌侵袭和转移的影响[J]. 实用癌症杂志, 2005, 20(2): 141-143 |
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| 作者姓名： | 潘国庆 金树珍 付红梅 边莉 唐慰萍 |
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| 作者单位： | 1. 650031,昆明医学院第一附属医院
2. 524001,广东医学院病理教研室 |
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| 摘 要： | 目的探讨尿激酶激活剂抑制剂-1(PAI-1)对鼻咽癌侵袭和转移的影响。方法以高低不同淋巴道转移能力的鼻咽癌细胞系(CNE-2Z)及其克隆株(CNE-2Z—H5，CNE-2Z—H5—9)作为研究材料；应用逆转录聚合酶反应(RT—PCR)方法检测PAI-1表达水平；应用酶联吸附法(ELISA)测定PAI-1在3种细胞中的蛋白表达量；通过侵袭抑制实验观察PAI-1在鼻咽癌的侵袭和转移过程中的作用。结果PAI-1在CNE-2Z、CNE-2Z—H5中均有表达，但无显著性差异；PAI-1在(2NE-2Z—H5—9中无表达。抗PAI-1抗体对CNE-2Z—H5—9侵袭无影响。结论PAI-1可能抑制鼻咽癌的侵袭和转移。
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| 关 键 词： | PAI-1 侵袭和转移 CNE-2Z 逆转录聚合酶反应 鼻咽癌细胞系 酶联吸附法 显著性差异 酶激活剂 转移能力 PCR） 转移过程 实验观察 抑制剂 克隆株 淋巴道 表达量 种细胞 |
| 文章编号： | 1001-5930(2005)02-0141-03 |
| 修稿时间： | 2004-11-13 |
Effect of PAI-1 on the Invasion and Metastasis in Nasopharyngeal Carcinoma |
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| PAN Guo-qing,JIN Shu-zhen,FU Hong-mei,et al.. Effect of PAI-1 on the Invasion and Metastasis in Nasopharyngeal Carcinoma[J]. The Practical Journal of Cancer, 2005, 20(2): 141-143 |
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| Authors: | PAN Guo-qing JIN Shu-zhen FU Hong-mei et al. |
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| Affiliation: | PAN Guo-qing,JIN Shu-zhen,FU Hong-mei,et al. The First Affiliated Hospital of Kunming Medical College,Kunming,650031 |
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| Abstract: | Objective To explore the effect of PAI-1 on the invasion and metastasis in Nasopharyngeal Carcinoma(NPC).Methods A Nasopharyneal carcinoma cell-line(CNE-2Z) and its sub-line(CNE-2Z-H5,CNE-2Z-H5-9) with different ability of lymph node metastasis were used.RT-PCR was used to detect the expression of PAI-1 at mRNA level.The expression of PAI-1 at protein level was measured by ELISA.The effect of PAI-1 on the invasion and metastasis was observed in the invasion inhibitory experiments.Results The PAI-1 was expressed in CNE-2Z,CNE-2Z-H5 with no significant difference between them(P>0.05),but was not expressed in CNE-2Z-H5-9.Anti-PAI-1 antibody didn't affect the invsion of CNE-2Z-H5-9.Conclusion PAI-1 may have an inhibitory effect on the metastasis and invasion in NPC. |
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| Keywords: | Plasminogen actioator inhibitor-1(PAI-1) Nasopharyngeal carcinoma(NPC) Invasion Metastasis Cell-line RT-PCR |
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