慢性乙型肝炎患者血清HBV DNA水平与肝组织病理及免疫组化关系探讨

目的:探讨慢性乙型肝炎患者血清HBV DNA水平与肝组织病理及免疫组化的关系。方法:随机选择98例慢性乙型肝炎患者,行肝活检术取肝组织病理诊断,采用免疫组织化学法,检测其中的HBsAg和HBcAg。结果:随着慢性乙型肝炎患者肝组织炎症活动度和纤维化程度的加重,血清HBV DNA水平呈中、高量分布,明显高于低水平组,两组间差异有显著性意义(P<0.01)。血清HBV DNA呈现高水平复制时,肝组织中HBsAg和HBcAg亦呈现显著表达,有较好的一致性。结论:慢性乙型肝炎患者肝组织中有较高的HBV复制率,其肝组织中HBsAg和HBcAg的分布类型与HBV复制状况和肝组织病变活动性有相关性。

Study on Relationship among Pathological and Immunohistochemistry Changes in Liver Tissues, and Level of HBV DNA in Serum of Patients with Chronic Hepatitis B

Objective: To study the relationship among pathological and immunohistochemistry changes in Liver tissues, and the level of HBV DNA in serum of the patients with chronic hepatitis B. Methods: 98 patients with chronic hepatitis B collected by random sampling methods. The pathological diagnosis in the liver tissues, the lever of HBV DNA in the serum were performed and the HBsAg and the HBcAg expression in the liver tissues were detected by irnmuno-cytochemistry technique. Results: As the liver tissue inflammation activity and liver fibrosis was elevation of the patients with chronic hepatitis B, the HBV DNA in the serum were correlated was significantly higher than low groups, there was significantly difference (P< 0.01). When HBV DNA in serum was in high replication statas, the significantly expression of HBsAg was coherent with that of HBcAg in the liver tissues. Conclusion: The results suggest that HBV replication rate were higher in the liver tissues of these patients. The expression of HBsAg and HBcAg in the liver were correlated with the HBV replication and the activity of liver tissue patholohical changes.