克湿灵治疗类风湿性关节炎的作用机制研究

目的:了解克湿灵(KSL)对大鼠佐剂性关节炎动物模型的作用机制。方法:氟氏完全佐剂诱发大鼠佐剂性关节炎;以炎性肿胀足的前列腺素E₂含量、细胞因子、淋巴细胞的功能变化作为观察指标;用紫外分光光度法测定炎性肿胀足的前列腺素E₂含量,采用MTT法检测胸腺、脾淋巴细胞增殖反应;用活化的小鼠脾淋巴细胞MTT比色法检测胸腺淋巴细胞产生的IL-2的活性。结果:克湿灵高(540 mg.kg^-1)、中(270 mg.kg^-1)、低(135 mg.kg^-1)3个剂量均能降低炎性肿胀足前列腺素E2的含量,其降低的量分别为25.6,16.1,10.0(A×1 000);克湿灵高、中2个剂量组可明显抑制刀豆蛋白A(ConA)诱导的T淋巴细胞增殖反应和IL-2的生成,体内给药对T淋巴细胞增殖反应的抑制率分别为32.1%,31.0%,对IL-2生成的抑制率分别为17.5%,14.0%;体外给药对T淋巴细胞增殖反应的抑制率分别为39.0%,22.1%,对IL-2生成的抑制率分别为27.3%,18.2%;脂多糖(LPS)诱导的B淋巴细胞增殖无明显变化。结论:克湿灵对类风湿性关节炎具有抗炎作用。

Study on mechanism underlying the treatment of rheumatoid arthritis by Keshiling

Objective: To explore the mechanism underlying the treatment of rheumatoid arthritis by Keshiling(KSL) in the rats model of FCA-induced arthritis.Method: The experimental arthritis was induced by FCA in the rats.The content of PGE₂ in the inflammatory swelling toes was evaluated by ultraviolet spectrophotometric method.The ConA and LPS-induced lymphocytes proliferation and the production of interleukin-2(IL-2) secreted by thymus were determined by MTT assay.Result: Results showed that the increases of lymphocyte proliferation and IL-2 production in AIA rats could be inhibited by KSL at the concentrations of 540 and 270 mg·kg^-1 in vivo and vitro.KSL at the same doses decreased the contents of PGE₂ in inflammatory swelling toes,and the decreased A values were 25.6,16.1,10.0(A×10³),respectively.After administration of KSL in vivo at 540 and 270 mg·kg^-1 the T lymphocyte proliferation were attenuated by 32.1% and(31.0%),and the production of IL-2 was inhibited by 17.5% and 14.0% respectively.While the inhibitory rates of T lymphocyte proliferation were reduced by 39.0% and 22.1% and the production of IL-2 was diminished by 27.3% and 18.2% respectively following the administration of KSL in vitro.Conclusion: KSL possesses the anti-inflammation function.