不同麻醉下电针对脑缺血再灌注大鼠脑能量代谢及氧供需平衡的影响

目的观察脑缺血再灌注损伤后大鼠颈静脉血糖、乳酸和血氧饱和度的变化,以及不同麻醉下电针对大鼠颈静脉血糖、乳酸和血氧饱和度的影响。方法雄性SD大鼠40只,随机分为5组,即:假手术组(SH)、水合氯醛+脑缺血再灌注组(CL+CI/R)、水合氯醛+脑缺血再灌注+电针组(CL+CI/R+EA)、异丙酚+脑缺血再灌注组(P+CI/R)、异丙酚+脑缺血再灌注+电针组(P+CI/R+EA),每组8只。采用4-VO法建立大鼠全脑缺血模型,于再灌流开始后,CI/R+EA组电针"百会""命门"和"足三里"穴,电针参数:频率30~50 Hz,间断疏密波,电流强度1 mA,以局部肌肉轻微震颤为度,时间20 min。在缺血后24 h,从右颈静脉抽取0.5 mL血样,进行血糖、乳酸和血氧饱和度的测定。结果CL+CI/R组大鼠的颈静脉血糖和血氧饱和度显著高于SH组(P0.01),乳酸值则低于SH组(P0.01),P+CI/R组大鼠的颈静脉血糖和血氧饱和度明显高于SH组(P0.05),乳酸值则显著低于SH组(P0.01);与CL+CI/R组相比,CL+CI/R+EA组和P+CI/R+EA组的颈静脉血糖和血氧饱和度显著降低(P0.01),而乳酸值则显著升高(P0.01);与P+CI/R组相比,实施电针治疗的P+CI/R+EA组的颈静脉血糖显著降低(P0.01),而乳酸值则明显升高(P0.05);P+CI/R组的颈静脉血糖明显低于CL+CI/R组的血糖(P0.05)。结论针刺治疗可明显改善脑缺血再灌注大鼠的糖代谢,增加脑细胞对葡萄糖和氧的摄取和利用,不同的静脉麻醉药物对电针治疗效果的影响无显著差异性。

Effects of Electroacupuncture on Cerebral energy metabolism and equilibrium of oxygen supply and demand of Rats with Cerebral Ischemia-Reperfusion Injury under different anesthesia

Objective To observe the changes of the jugular venous blood glucose,blood lactate and saturation of blood oxygen of rats with cerebral ischemia-reperfusion injury and the effects of electroacupuncture on levels of the jugular venous blood glucose,blood lactate and saturation of blood oxygen of the rats under different anesthesia.Methods Forty male SD rats were randomly divided into sham operation(SH),CL+CI/R,CL+CI/R+EA,P+CI/R and P+CI/R+EA groups with eight rats in each group.Transient general brain ischemia was induced by four-vessel occlusion and reperfusion.EA(30 to 50 Hz,1 mA) was applied to Baihui(GV20),Mingmen(GV4) and Zusanli(ST36) for 20 min after CI/R.Right jugular vein was cannulated to withdraw 0.5 mL blood samples for analysis blood gas,lactate and saturation of blood oxygenat the postischemia 24 h. Results The levels of the jugular venous blood glucose and saturation of blood oxygen after ischemia-reperfusion in CL+CI/R group increased while lactate decreased significantly than that in sham group(P<0.01);the levels of the jugular venous blood glucose and saturation of blood oxygen in P+CI/R group increased(P<0.05) while lactate decreased significantly than that in sham group(P<0.01);compared with CL+CI/R group,the levels of the jugular venous blood glucose decreased(P<0.01) while lactate increased remarkably in CL+CI/R+EA group and P+CI/R+EA group(P<0.01);compared with P+CI/R group,the levels of the jugular venous blood glucose decreased(P<0.01) while lactate increased remarkably in P+CI/R+EA group(P<0.05);the levels of the jugular venous blood glucose in P+CI/R group is significantly lower than that in CL+CI/R group(P<0.05).Conclusion The treatment with electropuncture can improve the glycometabolism of rats with cerebral ischemia-reperfusion injury and facilitate the brain cell to absorb and utilize glucose;and the different intravenous anesthetics have no effect on conduction of electroacupuncture signs.