血管紧张素转化酶底物的制备及对肺癌诊断的临床意义

目的 建立血管紧张素转化酶(ACE)底物的合成方法,探讨血清ACE活性的测定方法.方法 底物制备采用N-羟基琥珀酰亚胺活化酯合成多肽法;建立分光光度法测定ACE的活性.结果 底物呋喃丙烯酰苯丙氨酰甘氨酰甘氨酸纯度为99.5%.底物的米氏常数K_m为0.25 mmoL/L,血清ACE活性正常参考值范围46.7-113.3 U/L,标本批内和批间平均变异系数分别为3.9%和4.9%.临床检测表明,肺鳞癌、肺腺癌患者血清ACE活性显著下降(P<0.05);而肺小细胞癌患者ACE活性没有明显降低(P>0.05).结论 所合成的底物适用于临床诊断各类肺癌的要求.

Synthesis of substrate for angiotensin-converting enzyme and its value in diagnosis of lung cancer

Objective To establish the synthetic method of the substrate for angiotensin-converting enzyme(ACE),and investigate the assay method of serum ACE.Methods The substrate was prepared by the use of N-hydroxysuccinimide ester in peptide synthesis,and serum ACE was determined by spectrophotometric assay using substrate.Results The substrate of furanacryloyl-phenylalanyl-glycyl-glycine with purity of 99.5%was obtained.The K_m value for the substrate was 0.25 mmol/L,the normal reference interval for serum ACE acti...