骨骼肌桥接与神经移植对大鼠周围神经缺损的修复作用

目的：比较用半吻合、半埋入法骨骼肌桥接与神经移植治疗神经缺损的效果，以探讨半吻合、半埋入法骨骼肌桥接的临床应用价值。方法：选用体重200～300 g成年Wistar大白鼠15只，随机选择每只左侧后肢的坐骨神经造成的神经缺损用半吻合、半埋入法骨骼肌桥接，右侧神经缺损采用神经束膜缝合移植修复。大白鼠分笼饲养3个月。结果：3个月后，大白鼠双下肢功能均得到恢复，通过肉眼观察、手术显微镜下观察、神经肌电图检查及组织学检查，骨骼肌桥接侧与神经移植侧差异无显著性。半吻合、半埋入法骨骼肌桥接治疗周围神经缺损取得与神经移植相近的结果。结论：半吻合、半埋入法骨骼肌桥接手术难度低、时间短、吻合牢靠，是一种具有潜在临床应用价值的神经修复方法。

Effects of bridging by skeletal muscle and nerve graft on defect of peripheral nerves in Wistar rats

Objective To evaluate the effects of bridging by skeletal muscle(semi-anastomsois and semi-implantation method)and nerve graft on defect of the peripheral nerves,discuss the use of semi-anastomsois and semi-implantation method in clinic. Methods Fifteen Wistar rats (weight of 200～300 g) were selected to experiment. The sciatic nerves were sutured by semi-anastomsois and semi-implantation with skeletal muscle on left. At the same time, right common peroneael nerves were treated by the nerve graft, the changes of the two sides were measured after three months. Results After three months,the functions of two legs resumed.By macrography and observing under microscope,nerve electrophysiology,histology, there was no significant difference between two groups in the number regenerated fiber crossed sectional area. The effects of semi-anastomsois and semi-implantationwith skeletal muscle was as same as nerve graft. Conclusion The method of semi-anastomsois and semi-implantation with skeletal muscle not only operates easily but also saves time and inosculates reliably.It would be a potential method on restoring nerve defect .It has applicable value.