黄金牡丹合剂对内毒素血症小鼠细胞因子的影响

目的:研究黄金牡丹合剂(HJM)对内毒素血症小鼠细胞因子的影响。方法:腹腔注射内毒素(LPS)以复制小鼠内毒素血症模型。实验分为空白(等容无菌水)、模型对照(等容无菌水)、地塞米松(DEXA,0.80mL.kg-1)和HJM高、中、低剂量(1.00、0.50、0.25mL.kg-1)组,灌胃给药,每天2次,连续3d,观察以上6组小鼠72h死亡率。采用相同给药方式,以酶联免疫吸附法(ELISA)测定复制模型后空白、模型对照、DEXA和HJM中剂量组6、12、18、24、48、72h血清肿瘤坏死因子α(TNF-α)、白细胞介素(IL)-4、IL-6含量。结果:与模型对照组比较,HJM中剂量组死亡率显著降低(P<0.01)。HJM组TNF-α、IL-6含量显著降低(P<0.01或P<0.05),IL-4含量显著升高(P<0.05)。结论:HJM能显著降低内毒素血症小鼠死亡率,其机制可能与降低血清中TNF-α、IL-6的含量,促进IL-4释放,抑制过度炎症反应有关。

Effects of Huangjin Mudan Mixture on Cytokine of the Mice with Endotoxemia

OBJECTIVE:To study the effects of Huangjin mudan mixture(HJM)in treatment mice with endotoxemia.METHODS:Endotoxemia model was induced by intraperitoneal injection of endotoxin,then animals were divided into 6 groups,i.e.blank group(constant volume sterile water),model control group(constant volume sterile water),dexamethasone group(DEX,0.80 mL·kg-1)and HJM high-dose,medium-dose and low-dose groups(1.00 mL·kg-1,0.50 mL·kg-1,0.25 mL·kg-1).They were given relevant drug intragastrically twice a day for 3 days.72 h mortality of the six groups were observed.The same delivery mode.and ELISA assay of blank group,model control group,DEXA,HJM medium-dose was adopted to TNF-α,IL-4 and IL-6 levels in 6,12,18,24,48,72 h.RESULTS:Compared with model control group,the mortality of HJM medium-dose group decreased significantly(P<0.01).TNF-α and IL-6 of HJM group decreased significantly(P<0.01 or P<0.05),IL-4 content increased significantly(P<0.05).CONCLUSION:HJM can decrease the mortality of endotoxemia mice,and its mechanism is associated with decreasing the levels of TNF-αand IL-6,promoting the release of IL-4 and inhibiting excessive inflammatory response.