电针对局灶性脑缺血大鼠脑细胞内Ca2+含量的影响

目的：研究电针对局灶性脑缺血大鼠脑细胞内Ca^2+的调节作用。方法：利用激光共聚焦扫描显微镜观察缺血区活体脑片中脑细胞内Ca^2+的变化。结果：缺血1h，皮质部细胞内Ca^2+含量还未出现明显变化，而纹状体部细胞内Ca^2+的含量明显升高；缺血3h，皮质部和纹状体细胞内Ca^2+含量均出现显著的升高，且纹状体细胞内Ca^2+含量明显高于皮质部；缺血3h并给予电针治疗30min后，则皮质部和纹状体细胞内Ca^2+含量均出现明显的下降。结论：电针可迅速调节缺血区脑细胞内的Ca^2+含量，抑制胞内Ca^2+超载，从而保护脑缺血后继发神经元的损伤。

Effect of Electro-Acupuncture on Calcium Content in Neurocytes of Focal Cerebral Ischemia

Objective: To study the effect of electro acupuncture (EA) in regulating calcium (Ca 2 ) content in brain neurocytes of rats with focal cerebral ischemia. Methods: The changes of Ca 2 content in ischemic neurocytes were observed by using laser confocal scanning microscope. Results: Ca 2 content did not change significantly in cerebral cortex when the brain ischemia occurred for 1 hr, but it raised significantly in striatum neurocytes. Both the Ca 2 contents in striatum and cortex area increased significantly 3 hrs after ischemia occurrence and the content in striatum was higher than that in cortex significantly. Brain Ca 2 content could be reduced significantly after the 3 hrs ischemic brain were treated by EA for 30 min. Conclusion: EA could regulate the content of Ca 2 in the ischemic area of brain, inhibit Ca 2 overload, so as to protect neurons from ischemic injury.