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| 胃癌单抗与柔红霉素和氨甲喋呤交联物的制备及其细胞毒作用 | |
| 引用本文： | 高普军 郭晓林. 胃癌单抗与柔红霉素和氨甲喋呤交联物的制备及其细胞毒作用[J]. 中国免疫学杂志, 1993, 9(6): 352-354 |
| 作者姓名： | 高普军 郭晓林 |
| 作者单位： | 白求恩医科大学第一临床学院消化内科 长春130021(高普军，郭晓林，宋国培)，白求恩医科大学第一临床学院消化内科 长春130021(朴云峰) |
| 摘 要： | 采用葡聚糖氧化将胃癌单抗(McAb)与抗癌药物柔红霉素(DNR)和氨甲喋呤(MTX)同时交联(McAb-PAD<分别采用噻唑蓝活细胞染色法(MTT 法)及~3H-TR 掺入法检测交联物的细胞毒作用,结果表明两种检测方法敏感性无明显差异,并且证实文联物对肿瘤靶细胞具有较强的选择性杀伤效应。 |
| 关 键 词： | 单克隆抗体 癌 导向疗法 胃肿瘤 |
Preparation of anti-gastric cancer monoclonal antibody with daunorubicin methotrexate conjugate and its cytotoxiciy to gastric cancer cells. |
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| GAO Pu-jun GUO Xiao-lin SONG Guo-pei PIAO Yun-feng. First Teaching Hospital Norman Bethune University of Medical Sciences,Chanchun. Preparation of anti-gastric cancer monoclonal antibody with daunorubicin methotrexate conjugate and its cytotoxiciy to gastric cancer cells.[J]. Chinese Journal of Immunology, 1993, 9(6): 352-354 | |
| Authors: | GAO Pu-jun GUO Xiao-lin SONG Guo-pei PIAO Yun-feng. First Teaching Hospital Norman Bethune University of Medical Sciences Chanchun |
| Affiliation: | GAO Pu-jun GUO Xiao-lin SONG Guo-pei PIAO Yun-feng. First Teaching Hospital Norman Bethune University of Medical Sciences,Chanchun 130021 |
| Abstract: | It is first time to use dextren T-40 oxidative method to conjugate anti-gastric cancer mono-colonal antibody(McAb)with anti-tumor medicines of daunorubicin(DNR)and methotrexate(MTX)together.Cytotoxicity of conjugates was measured by MTT method and ~3H-TdR incor-poration method respectively.Both sensitivity is similar.The results have showed that this conju-gate exhibited selective cytotoxicity on human gastric cancer cells in vitro. |
| Keywords: | McAb Gastric cancer DNR MTX Targeted treatment |
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