癌胚抗原的提取和纯化

本文报道从结肠癌肝转移灶中提取和纯化癌胚抗原的步骤。肝转移组织制成匀浆,用过氯酸和乙醇提取得到癌胚抗原的粗制品,经sepharose 4B和sephadex G-200 凝胶过滤,Con A琼脂糖亲和层析进行纯化。所得产品用 SDS凝胶电泳、琼脂扩散、免疫电泳和放射免疫分析法等鉴定,分子量为200,000,免疫活性与法国提供的癌胚抗原和国际参考制剂相一致,剂量校对100ng=1国际单位。

THE ISOLATION AND PURIFICATION OF CARCINOEMBRYONIC ANTIGEN

The Procedures of isolation and purification of the carcinoembryonic antigen from hepatic metastatic tissue of colonic carcinoma were presented. The crude CEA obtained by the extraction of hepatic homogenate with perchloric acid and ethyl alcohol. Purification were carried out by sepharose 4B, Sephadex G200 gel filtration and Con A sepharose affinity chromatography. The final product, then identified by SDS gel electrophoresis, agar diffusion, immuno electrophoresis and radioimmunoassay. It was demonstrated that the present CEA has a molecular weight of 200,000, its immunoactivity was as same as the French CEA and the International reference preparation, and dose calibration was found to be 100ng correspodence to 1 international unit.