新疆维、汉族宫颈鳞癌病变与 PI3K 、ERK 、IG F-1R、ER蛋白表达的关系

目的：从蛋白质水平观察子宫颈炎和子宫颈鳞癌组织中磷酸肌醇3激酶（PI3K ）、细胞外信号调节激酶（ERK ）、胰岛素样生长因子‐1型受体（IGF‐1R）和雌激素受体（ER）在新疆维吾尔族和汉族妇女中的表达及相关关系。方法收集石蜡包埋宫颈炎组织90份（汉族46份，维吾尔族44份）、子宫颈鳞癌组织224份（汉族36份，维吾尔族188份），采用免疫组织化学S P法鉴定蛋白表达水平。结果子宫颈鳞癌IGF‐1R、PI3K阳性表达率（58．04％、92．41％）高于宫颈炎组织（13．33％、57．78％），ER、ERK阳性表达率（22．32％、68．30％）低于宫颈炎组织（63．33％、95．56％）；汉族、维吾尔族子宫颈鳞癌IGF‐1R和PI3K阳性表达率（69．44％和88．89％，55．85％和93．09％）高于宫颈炎组织（15．22％和54．35％，11．36％和61．36％）；汉族、维吾尔族子宫颈鳞癌ER和ERK阳性表达率（13．89％和83．33％，23．94％和65．43％）低于宫颈炎组织（65．22％和93．48％，61．36％和97．73％）；ERK在维吾尔族子宫颈鳞癌组织中表达（65．43％）低于汉族（83．33％），差异均有统计学意义（ P＜0．01）。结论 PI3K、ERK、IGF‐1R和ER蛋白表达阳性或缺失与宫颈癌发生关系密切，可作为宫颈癌检测的重要生物指标，ERK蛋白在宫颈癌中的表达存在民族间差异。

Relation between cervical squamous cancer lesion among Uighur and Han ethnic women in Xinjiang with expression of PI3K,ERK,IGF-1R and ER protein

Objective To observe the expression levels of PI3K ,ERK ,IGF‐1R and ER in cervicitis and cervical squamous cancer tissues among Uighur and Han ethnic patients and their correlation .Methods The 90 paraffin embedding samples of cervici‐tis tissue( 46 cases for Han and 44 cases for Uighur) and 224 paraffin embedding samples of cervical squamous cancer tissue (36 ca‐ses for Han and 188 cases for Uighur) were collected and detected the protein expression levels by using immunohistochemistry .Re‐sults The positive expression rates of IGF‐1R and PI3K in cervical squamous cancer were 58 .04% and 92 .41% respectively ,which were higher than 13 .33% and 57 .78% in cervicitis tissue ,the positive expression rates of ER and ERK in cervical squamous cancer were 22 .32% and 68 .30% respectively ,which were lower than 63 .33% and 95 .56% in cervicitis tissue ;the positive expression rate of IGF‐1R and PI3K of cervical squamous cancer in Han and Uighur were 69 .44% ,88 .89% and 55 .85% ,93 .09% respective‐ly ,which were higher than 15 .22% ,54 .35% and 11 .36% ,61 .36% of cervicitis tissue ;the positive expression rate of ER and ERK of cervical squamous cancer in Han and Uighur were 13 .89% ,83 .33% and 23 .94% ,65 .43% respectively ,which were lower than 65 .22% ,93 .48% and 61 .36% ,97 .73% of cervicitis tissue respectively ;the expression of ERK in Uighur cervical squamous carci‐noma tissue was 65 .43% ,which was lower than 83 .33% in Han ,the difference was statistically significant (P<0 .01) .Conclusion PI3K ,ERK ,IGF‐1R and ER protein expression positive or deficiency is closely related to the occurrence of cervical cancer ,which may serve as the important biological indicators for detecting cervical cancer ,and the ethnic difference of ERK protein expression exists in cervical cancer .