胆木枝叶的化学成分研究

目的研究茜草科乌檀属植物胆木Nauclea officinalis枝叶中的化学成分。方法综合运用硅胶柱色谱、反相硅胶柱色谱和Sephadex LH-20凝胶柱色谱以及制备型高效液相等色谱方法进行系统分离,根据化合物的理化性质及其波谱数据确定化合物的结构。结果从胆木85%乙醇提取物中分离得到了18个化合物,分别鉴定为二氢猕猴桃内酯(1)、黑燕麦内酯(2)、3,4,5-三甲氧基苯酚(3)、4-羟基-3,5-二甲氧基苯甲醛(4)、2,4-二羟基-3,6-二甲基苯甲酸甲酯(5)、对甲氧基桂皮酸(6)、咖啡酸甲酯(7)、咖啡酸乙酯(8)、异阿魏酸甲酯(9)、阿魏酸乙酯(10)、断氧化马钱子苷(11)、裂环马钱苷(12)、3-醛基吲哚(13)、1,2,3,4-tetrahydronorharman-1-one(14)、vinmajine I(15)、19-O-methyl-3,14-dihydroangustoline(16)、乌檀碱(17)和异长春花苷内酰胺(18)。其中化合物1、2为倍半萜类化合物,3~10为酚酸类化合物,11、12为环烯醚萜类化合物,化合物13~18为生物碱类化合物。结论化合物1~15为首次从茜草科乌檀属植物中分离得到。

Chemical constituents from stems and leaves of Nauclea officinalis

Objective To study the chemical constituents from stems and leaves of Nauclea officinalis. Methods The chemical constituents from N. officinalis were separated and purified by silica gel, ODS, Sephadex LH-20 gel column chromatographies, and preparative HPLC. Their structures were identified by physicochemical properties, spectroscopic analysis, as well as comparisons with the data in literature. Results Eighteen compounds were isolated from 85% ethanol extract from N. officinalis, and identified as dihydroactinidiolide (1), loliolide (2), 3,4,5-trimethoxyphenol (3), 4-hydroxy-3,5-dimethoxybenzaldehyde (4), methyl-2,4-dihydroxy-3,6-dimethyl-benzoate (5), p-methoxy cinnamic acid (6), caffeic acid methyl ester (7), ethyl caffeate (8), methyl isoferulate (9), ethyl ferulate (10), secoxyloganin (11), secologanoside (12), 1H-indole-3-aldehyde (13), 1,2,3,4-tetrahydronorharman-1-one (14), vinmajine I (15), 19-O-methyl-3,14-dihydroangustoline (16), naucleidinal (17), and strictosamide (18). Conclusion Compounds 1-15 were isolated from the genus Nauclea Linn. for the first time.