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| 过表达IL-18的T细胞对胰腺癌细胞SW-1990的杀伤作用 | |
| 引用本文： | 钟根深,尤朵,陈亚楠,杨如,许芝山,吴敏娜. 过表达IL-18的T细胞对胰腺癌细胞SW-1990的杀伤作用[J]. 基础医学与临床, 2017, 37(9). DOI: 10.3969/j.issn.1001-6325.2017.09.011 |
| 作者姓名： | 钟根深 尤朵 陈亚楠 杨如 许芝山 吴敏娜 |
| 作者单位： | 1. 新乡医学院 第一附属医院 生命科学技术研究中心,河南 卫辉,453100;2. 新乡医学院 基础医学院 微生物学教研室,河南 新乡,453003 |
| 基金项目： | 国家自然科学基金青年项目，河南省高校重点科研项目，新乡医学院研究生科研创新支持计划 |
| 摘 要： | 目的探讨IL-18基因过表达的T细胞对胰腺癌细胞系SW1990的杀伤作用。方法用PCR技术构建含IL-18基因的重组慢病毒,HEK293T细胞包装后感染分离培养的人T淋巴细胞,与胰腺癌细胞SW-1990共培养后,检测培养上清中的LDH含量、ELISA检测IL-2和IFN-γ的含量,以间接评估其对SW-1990细胞体外的杀伤作用。结果成功构建和包装了人IL-18的重组慢病毒,感染人T淋巴细胞后,在与人胰腺癌细胞SW-1990共培养条件下,与空白对照组相比,LDH的释放显著增多(P0.01);IL-2和IFN-γ的分泌亦显著增多(P0.01)。结论过表达人IL-18蛋白的T淋巴细胞对胰腺癌细胞SW-1990具有更强的杀伤作用。 |
| 关 键 词： | 胰腺癌 IL-18 T淋巴细胞 |
Overexpression of IL-18 gene by T lymphocytesenhances their cytotoxic effect against pancreatic cancer cells SW-1990 |
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| ZHONG Gen-shen,YOU Duo,CHEN Ya-nan,YANG Ru,XU Zhi-shan,WU Min-na. Overexpression of IL-18 gene by T lymphocytesenhances their cytotoxic effect against pancreatic cancer cells SW-1990[J]. Basic Medical Sciences and Clinics, 2017, 37(9). DOI: 10.3969/j.issn.1001-6325.2017.09.011 | |
| Authors: | ZHONG Gen-shen YOU Duo CHEN Ya-nan YANG Ru XU Zhi-shan WU Min-na |
| Abstract: | Objective To investigate the antitumor effects of IL-18 gene transfected T cells on pancreatic cancer cell SW-1990.Methods Construction of IL-18 contained recombinant lentivirus using PCR method and packaged at HEK293T cell, then transfected the human T cells and evaluated the antitumor effects when cocultured with SW-1990.Results The IL-18 gene contained recombinant lentivirus was successfully constructed and packaged, and transfected the T cells, the LDH secretion and IL-2 and IFN-γ content all increased significantly (P<0.01) when cocultured with SW-1990 cell.Conclusions T cells transfected with IL-18 possessed more potent antitumor effects to pancreatic cancer cell SW-1990 as compared to the regular T cells. |
| Keywords: | pancreatic cancer IL-18 T cell |
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