PTPN13在人胃癌组织和胃癌SGC-7901细胞系中的表达及其对细胞增殖和侵袭的影响

目的分析PTPN13在人胃癌组织中及胃癌SGC-7901细胞系中的表达及其对细胞增殖、侵袭的影响。方法收集106例胃癌患者的胃癌组织及癌周正常组织标本。常规培养SGC-7901细胞并分为pc DNA3.1-PTPN13质粒转染组和未转染组。免疫组织化学法检测组织中的PTPN13表达;分析PTPN13表达与患者性别、年龄、肿瘤部位、肿瘤大小、浸润深度及转移的关系;Kaplan-Meier曲线分析不同PTPN13表达患者生存率的差异;CCK-8法检测细胞的增殖能力;Trans-well试验分析侵袭能力;Western blot检测E-cadherin、Snail及MMP9的表达。结果胃癌组织中的PTPN13阳性率低于癌周正常胃组织(31%vs 83%,P0.05);PTPN13的表达与肿瘤直径、浸润深度、淋巴结和远隔器官转移情况有关(P0.05);PTPN13阴性的胃癌患者2生存率较低;PTPN13过表达可以降低SGC-7901细胞的增殖率(P0.05),同时降低其侵袭能力(P0.05);上调PTPN13后SGC-7901细胞上皮化标志物E-cadherin表达增加,而间质化标志物Snail和MMP9表达减少。结论 PTPN13在胃癌组织和胃癌细胞中具有肿瘤抑制作用,较低的PTPN13表达提示患者预后不良。PTPN13具有成为胃癌的治疗诊断或治疗靶点的潜力。

Expression of PTPN13 in human gastric cancer and gastric cancer SGC-7901 cell line and its effect on cell proliferation and invasion

Objective To investigate the expression of PTPN13 in human gastric cancer and gastric cancer SGC-7901celllineanditsassociationwithproliferationandinvasion.Methods 106casesgastriccancertissuesamples and matched normal peritumorial tissues were collected .SGC-7901 cells were cultured and divided into two groups including pcDNA3.1-PTPN13 transfection group and without transfection group .Immunohistochemical technique was used to detecte protein expression of PTPN 13.The association of PTPN13 expression with tumor location , tumor size , depth of invasion and tumor metastasis were analyzed .The survival rate of patients with different PT-PN13 expression was calculated by Kaplan-Meier curves.CCK-8 assay was used to estimate the proliferation chan-ges.Using Trans-well assay analyzed the invasion of SGC-7901 cells.Moreover, Western blot was performed to de-tect the markers of EMT including E-cadherin, Snail and MMP9.Results Immunohistochemistry showed the expression rate of PTPN13 in gastric cancer tissues was lower than normal tissue (31 %vs 83%, P<0.05).The expression of PTPN13 in patients was related to with different tumor size , depth of invasion and tumor metastasis (P<0.05).The 2-year survival rate of patients with negative PTPN13 expression were lower.Overexpression reduced both proliferation and invasion in SGC-7901 cells.Up-regulated PTPN13 may increase E-cadherin level but decreases the level of Snail and MMP9 .Conclusions PTPN13 plays a role in gastric cancer tissue and cells as a tumor suppressor.Lower PTPN13 may predict a poor prognosis.PTPN13 may be used as therapy target in gastric cancer.