神经生长因子受体在先天性巨结肠表达的意义

目的 探讨神经生长因子受体（ＮＧＦＲ）在先天性巨结肠（ＨＤ）中的分布情况及其意义。方法 应用ＮＧＦＲ免疫组化方法对１０例３周￣２岁的ＨＤ患儿及８例对照组患儿结肠进行染色观察。结果 丰富的ＮＧＦＲ染色阳性神经纤维分布于正常结肠环肌层及粘膜下层，少量分布于纵肌层，ＮＧＦＲ染色阳性神经元分布于肌间神经丛及粘膜下神经丛；ＮＧＦＲ染色阳性神经纤维在ＨＤ无肌间神经节细胞肠段肌层及粘膜下层内明显减少或缺如，而肌

The Implication of Nerve Growth Factor Receptor Expression in Hirschsprung's Disease

Objective To evaluate the distribution of nerve growth factor receptor (NGFR) in Hirschsprung's disease (HD) and its clinical implication.Methods Ganglionic and aganglionic colonic specimens from 10 children with HD and normal colonic specimens from 8 controls were immuno stained with NGFR antibody.Results Extensive NGFR staining was found in the nerve fibers of muscularis and mucosae, neural tissue of the submucosal and myenteric plexuses of normal colon. NGFR positive nerve fibres were markedly reduced or absent in muscular layer and submucous layer while the hypertrophic nerve trunks in myenteric and submucosal plexuses showed a sheath of intense staining in the aganglionic colon from patients with HD.Conclusions The abnormal distribution of NGFR in the aganglionic bowel suggests involvement of NGFR in the pathogenesis of HD. NGFR immuno staining may be a valuable diagnostic tool in HD.