Thirteen single-nucleotide polymorphisms (SNPs) in the alcohol dehydrogenase 4 (ADH4) gene locus |
| |
| Authors: | A. Iida S. Saito A. Sekine K. Kondo C. Mishima Y. Kitamura S. Harigae S. Osawa Y. Nakamura |
| |
| Affiliation: | (1) Laboratory for Genotyping, RIKEN SNP Research Center, c/o Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan Tel. +81-3-5449-5716; Fax +81-3-5449-5718 e-mail: ariiida@ims.u-tokyo.ac.jp, JP;(2) Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo, Japan, JP |
| |
| Abstract: | The human alcohol dehydrogenase 4 (ADH4) gene encodes the class II ADH4 pi subunit, which contributes to the metabolization of a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Here we report the results of systematic screening for single-nucleotide polymorphisms (SNPs) in the ADH4 gene by means of direct sequencing combined with a polymerase chain reaction method. A total of 16 genetic variations including 13 SNPs were found; 4 in the 5′ flanking region, 4 in the 5′ untranslated region, and 8 within introns. No variation was found in coding, 3′ untranslated, or 3′ flanking regions. Eight of the 13 SNPs were not reported in the NCBI dbSNP database or any previous publications. Our SNP map presented here should provide tools to evaluate the role of ADH4 in complex genetic diseases and a variety of pharmacogenetic effects. Received: September 26, 2001 / Accepted: October 11, 2001 |
| |
| Keywords: | Single-nucleotide polymorphisms (SNPs) Insertion– deletion polymorphisms High-density SNP map Alcohol dehydrogenase 4 (ADH4) gene Japanese population |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
