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| HPLC法测定碱制斑蝥中斑蝥素的含量 | |
| 引用本文： | 刘艳芳,赵丽娜,张振凌. HPLC法测定碱制斑蝥中斑蝥素的含量[J]. 中医药学刊, 2010, 0(3): 487-488 |
| 作者姓名： | 刘艳芳 赵丽娜 张振凌 |
| 作者单位： | 河南中医学院,河南郑州450008 |
| 基金项目： | 中国药典2010版一部标准课题（YD-005） |
| 摘 要： | 目的：建立高效液相法测定碱制斑蝥中斑蝥素含量的方法。方法：采用Agilent HC-C18 ODS色谱柱（4.6mm×250mm）;流动相：甲醇-水（30∶70）;流速1.0mL/min;检测波长230nm,柱温：35℃。结果：碱制斑蝥中斑蝥素含量2%左右,远高于《中国药典》采用气相法测定斑蝥中斑蝥素含量不得少于0.35%的规定。结论：本法简便、快速、精确、重现性好,斑蝥药材中可能本来就含有斑蝥酸盐。 |
| 关 键 词： | 碱制斑蝥 斑蝥素 高效液相色谱法 |
Determination of Cantharidin in Mylabris After Processing with Potash by HPLC |
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| LIU Yan-fang,ZHAO Li-na,ZHANG Zhen-ling. Determination of Cantharidin in Mylabris After Processing with Potash by HPLC[J]. Study Journal of Traditional Chinese Medicine, 2010, 0(3): 487-488 | |
| Authors: | LIU Yan-fang ZHAO Li-na ZHANG Zhen-ling |
| Affiliation: | (Henan University of TCM,Zhengzhou 450008,Henan,China) |
| Abstract: | Objective：To establish an HPLC method for the determination of cantharidin in Mylabris after processing with potash. Methods：RP-HPLC was used in quantitative analysis the Agilent HC-C18 ODS Column（4.6mm×250mm） was used,and mobile phase was composed of methanol-water（30∶70）.Detection wavelength was at 230nm,the flow rate was 1.0mL/min,the column temperature was 35℃. Results：The determination of cantharidin in Mylabris after processing with potash is about 2%,the content of cantharidin in Mylabris is much higher than the regulations that it must not be less than 0.35% determined by gas chromatography in ＂chinese pharmacopoeia＂. Conclusion：The method was proved to be simple,rapid,accurate and reproducible,Mylabris phalerata Pallas may already contain cantharidate. |
| Keywords: | Mylabris atfer processing with potash cantharidin HPLC |
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