心气虚证模型大鼠脑内细胞凋亡相关基因的变化研究

目的：建立心气虚证大鼠模型,研究模型大鼠脑神经元细胞凋亡情况。方法：75只SD大鼠随机分为5组：正常对照组、疲劳对照组、小剂量心得安＋疲劳组、中剂量心得安＋疲劳组和大剂量心得安＋疲劳组,采用强迫跑步加灌服心得安的方法建立心气虚证大鼠模型,观察其脑内凋亡相关基因Bax、Bcl-2和Caspase-3 mR-NA的表达情况。结果：中剂量心得安＋疲劳组大鼠的大脑皮层和海马Bcl-2 mRNA表达明显降低;大剂量心得安＋疲劳组大鼠的大脑皮层Bcl-2 mRNA表达明显降低、海马Caspase-3 mRNA表达明显升高。结论：心气虚证模型大鼠脑内存在神经元细胞的凋亡。

The Expression of Apoptosis-Related Gene in the Brain of Heart-Qi Deficiency （HQD） Syndrome Model Rats

Objective： To establish heart-Qi deficiency syndrome rat model and observe the expression of apoptosis-related gene in the brain of the model rats.Methods： Seventy-five SD rats were randomly divided into 5 groups： normal group（N）,exercise-induced fatigue group（F）,small dose propanolol＋exercise-induced fatigue group（SF）,middle dose propanolol＋exercise-induced fatigue group（MF） and great dose propanolol＋exercise-induced fatigue group（LF）.The rat models of heart-Qi deficiency syndrome were established by using complex factors including forced running till exhaust and gavaged propanolol,and the expressions of Bax mRNA,Bcl-2 mRNA and Caspase-3 mRNA in the rats brain were measured.Results： In group MF,the expression of Bcl-2 mRNA in the rats cortex and hippocampus descended obviously.In group LF,the expression of Bcl-2 mRNA in the rats hippocampus decreased distinctly and the expression of Caspase-3 mRNA increased obviously.Conclusion： The neuron apoptosis exists in the brain of HQD syndrome model rats.