大鼠自体异体表皮细胞悬液混合移植的实验研究

目的　探讨自、异体表皮细胞悬液混合移植技术在创面修复中的应用。　方法　 30只大鼠随机配成 15对后 ,分成细胞悬液移植组 (A组 ,10对 )和细胞膜片移植组 (B组 ,5对 )。取每只大鼠全厚皮 ,分离表皮细胞 ,并根据配对情况按 1∶1的细胞比例混合 ,体外常规培养。 4d后收获A组混合细胞悬液 ,14d后收获B组混合细胞膜片。将此细胞悬液和膜片分别转移至A、B组相应供体大鼠的去全厚皮创面。随后A组每对大鼠的创面交叉覆盖配对方的异体全厚皮 ;B组创面覆盖胶原膜及“优妥”敷料。比较移植后 2～ 3周两组的创面修复情况。　结果　术后 2～ 3周 ,A组创面大多愈合 ,表面光滑 ,与皮下连接紧密。术后第 5天 ,B组创面部分细胞膜片脱落 ,部分成活 ,膜片成活的创面后期再次出现小创面 ,经久不愈。　结论　自、异体表皮细胞悬液混合移植是一种可行的、体内构建皮肤、修复创面的方法。

An experimental study on the in vivo intermingled culture of rat autologous and allogeneic epithelial cells

OBJECTIVE: To explore a method for in vivo skin reconstruction. METHODS: Thirty Sprague-Dawley (SD) rats were randomly divided into two paired groups, i.e. in vivo culture (A group, 10 pairs) and in vitro grafting (B group, 5 pairs). Skin samples were harvested from the rats of the two groups for the isolation of epithelial cells which were then mixed cultured in vitro in 1:1 ratio. Mixed cellular suspension in A group was harvested 4 days after culture. The mixed cellular sheets were harvested 14 days after culture. The cultured cells and sheets were then transplanted onto total skin loss wounds of donor rats for further cultivation. The wounds in A group were covered with allogeneic full-thickness skin. While the wounds in B group were covered by collagen membrane and gauze. Wound repair was observed and compared between the two groups at 2 - 3 post-operative weeks. RESULTS: Most of the wounds in A group healed after 2 - 3 weeks with smooth surface, and the peithelium connected closely and tightly with the subcutaneous tissue. In the wounds in B group on 5 post-operative day, some of the cellular sheets survived and some fell off. Even the healed wounds in B group would be injured again resulting in protracted small wounds. CONCLUSION: In vivo in situ epithelial culture might be an optional method of skin reconstruction for wound healing.