Mouse models in burns research: Characterisation of the hypermetabolic response to burn injury |
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| Affiliation: | 1. Burns Research and Reconstructive Surgery, ANZAC Research Institute, Concord Hospital, University of Sydney, Sydney, NSW, 2139, Australia;2. Adrenal Steroid Laboratory, ANZAC Research Institute, Concord Hospital, University of Sydney, Sydney, NSW, 2139, Australia;3. Bone Biology Group, ANZAC Research Institute, Concord Hospital, University of Sydney, Sydney, NSW, 2139, Australia;4. Burns and Reconstructive Surgery Unit, Concord Repatriation General Hospital, Concord, NSW, 2139, Australia;1. Burn Injury Research Unit, School of Surgery, University of Western Australia, Perth, Western Australia, Australia;2. Telethon Kids Institute, University of Western Australia, Perth, Western Australia, Australia;3. Fiona Wood Foundation, Perth, Western Australia, Australia;4. Burns Service of Western Australia, WA Dept. of Health, Perth, Western Australia, Australia;1. COREMED – Cooperative Centre for Regenerative Medicine, JOANNEUM RESEARCH Forschungsgesellschaft mbH, Graz, Austria;2. Division of Plastic, Aesthetic and Reconstructive Surgery, Department of Surgery Medical University of Graz, Graz, Austria;1. Department of Surgery, Rio de Janeiro State University, Boulevard Vinte e Oito de Setembro, 77, Vila Isabel, Rio de Janeiro, RJ 20551-030, Brazil;2. Department of Histology and Embryology, Rio de Janeiro State University, Av. Marechal Rondon, 381/HLA, São Francisco Xavier, Rio de Janeiro, RJ 20950-003, Brazil;1. Laboratory of Experimental Plastic, Reconstructive and Aesthetic Surgery, Faculty of Medicine, University of Geneva, Geneva, Switzerland;2. Department of Plastic, Reconstructive, Aesthetic and Hand Surgery, University Hospital of Basel (USB), Basel, Switzerland;3. Department of Pathology, University Hospitals of Geneva (HUG), Geneva, Switzerland;4. Department of Plastic Surgery and Hand Surgery, University Hospital of Zurich (USZ), Zurich, Switzerland;5. Department of Plastic, Reconstructive and Aesthetic Surgery, Ospedale Regionale di Lugano, Ente Ospedaliero Cantonale (EOC), Lugano, Switzerland;6. Clinic Utoquai, Zürich, Switzerland;7. Faculty of Medicine, University of Zurich, Zurich, Switzerland;1. Institute of Nuclear Medicine & Allied Sciences, Defence Research and Development Organization, Lucknow Road, Timarpur, Delhi 110054, India;2. Defence Institute of Physiology and Allied Sciences, Defence Research and Development Organization, Lucknow Road, Timarpur, Delhi 110054, India;1. Department of Medical Sciences, Clinical Physiology, Uppsala University, Uppsala, Sweden;2. Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden;3. Intensive Care Medicine, Department of Anesthesiology, Intensive Care Medicine and Pain Medicine, University of Helsinki and Helsinki University Hospital, Helsinki, Finland;4. Burn Center, Department of Plastic and Maxillofacial Surgery, Uppsala University Hospital, Uppsala, Sweden;5. Department of Surgical Sciences, Anaesthesiology and Intensive Care Medicine, Uppsala University, Uppsala, Sweden;6. The Hedenstierna Laboratory, CIRRUS, Department of Surgical Sciences, Anaesthesiology and Intensive Care Medicine, Uppsala University, Uppsala, Sweden;7. Department of Surgical Sciences, Plastic Surgery, Uppsala University, Uppsala, Sweden |
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| Abstract: | ObjectiveThe aim of the study is to characterise burn induced hypermetabolism in a mouse model.Summary Background DataThere are many mouse models of burn injury currently available however, their use in burns research is limited by the general assumption that post-burn hypermetabolism is difficult to study in these models.MethodsMale Balb/c mice were subjected to either a small (1 cm2) or large (4 cm2) contact burn. The hypermetabolic response to burn injury was determined by measuring changes in basal energy expenditure. The hormonal and inflammatory mediators of hypermetabolism, and the catabolic alterations secondary to hypermetabolism were also examined.ResultsPost-burn hypermetabolism was induced in both models of small and large burn. However, large burns resulted in prolonged wound healing, a more pronounced and sustained increase in basal energy expenditure, and a greater stress and systemic inflammatory response with profound catabolic consequences.ConclusionsIn the present study, we have successfully characterised the burn induced systemic hypermetabolic response in a mouse model of small and large burn. These models may prove useful for researchers studying the complex aetiology of hypermetabolism and interventions. |
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| Keywords: | Burns injury Hypermetabolism Mouse model Wound healing Inflammation Metabolism |
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