Higher expression of miR-150-5p promotes tumorigenesis by suppressing LKB1 in non-small cell lung cancer |
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| Affiliation: | 1. Lab for Noncoding RNA & Cancer, School of Life Sciences, Shanghai University, Shanghai 200444, China;2. School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China;3. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;4. QianWeiChang College, Shanghai University, Shanghai 200444, China;5. Shanghai Chest Hospital, 241 West Huaihai Road, Shanghai, China;1. Pathology Unit, Department of Advanced Biomedical Sciences, University of Naples “Federico II”, Naples, Italy;2. Gynecology and Obstetrics Unit, Department of Neurosciences, Reproductive Sciences and Dentistry, University of Naples “Federico II”, Naples, Italy;1. Cancer Research Center, Yanbian University Medical College, Yanji 133002, China;2. Department of Biochemistry and molecular biology, Yanbian University Medical College, Yanji 133002, China;3. Key laboratory of the Science and Technology Department of Jilin Province, Yanji 133002, China;1. Department of Pathology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea;2. Division of Gastroenterology, Department of Internal Medicine and Gastrointestinal Cancer Center, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea;3. Department of Surgery, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea;1. Department of Pathology, The First Affiliated Hospital of Xinjiang Medical University, No. 137 Liyushan Southern Road, Urumqi, The Xinjiang Uygur Autonomous Region of China, 830054, PR China;2. Xinjiang Medical University, No. 393 Xinyi Road, Urumqi, The Xinjiang Uygur Autonomous Region of China, 830011, PR China;3. Department of Pathology, Affiliated Traditional Chinese Medicine Hospital of Xinjiang Medical University, No. 116 Huanghe Road, Urumqi, The Xinjiang Uygur Autonomous Region of China, 830000, PR China |
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| Abstract: | ![]()
Lung cancer is one of the most malignant tumors that can form in the human. MicroRNAs (MiRNAs) play significant role in tumor progression. Human lung cancer tissues and cell lines were used to determine miR-150-5p respectively, and Liver Kinase B1 (LKB1) expression using quantitative real-time PCR (qRT-PCR). The data analysis website Kaplan-Meier Plotter (database obtained from The Cancer Genome Atlas) was used to perform a survival analysis with LKB1 levels. Using the appropriate assays, the function of miR-150-5p was also detected in cellular proliferation, migration and cell apoptosis as well as cell cycle. Results revealed that miR-150-5p was upregulated in non-small cell lung cancer (NSCLC) tissue and cell lines. In NSCLC, miR-150-5p promoted cellular proliferation and migration, but decreased cellular apoptosis. Conversely, miR-150-5p inhibition suppressed cellular growth. These results further revealed a network of cellular signaling for miR-150-5p to target LKB1. Ectopic expression of LKB1 can mitigate the tumor-promoting function of miR-150-5p. Collectively, these results indicated that miR-150-5p may promote lung cancer by inhibiting the suppressor gene LKB1 in NSCLC. |
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| Keywords: | miR-150-5p Cell growth Oncogene NSCLC LKB1 |
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