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| 丙型肝炎病毒F蛋白上调肝癌细胞HepG2中Bcl-2和Bax表达 | |
| 引用本文： | 韦娟,孔晶,邓小昭,储春丽,余晓杰,岳明,张云,徐孝东.丙型肝炎病毒F蛋白上调肝癌细胞HepG2中Bcl-2和Bax表达[J].疾病控制杂志,2012,16(4):289-292. |
| 作者姓名： | 韦娟 孔晶 邓小昭 储春丽 余晓杰 岳明 张云 徐孝东 |
| 作者单位： | 1. 南京医科大学基础医学院生物化学与分子生物学系,江苏南京,210029 2. 淮阴工学院生命科学与化学工程学院,江苏淮安,223300 3. 南京医科大学基础医学院生物化学与分子生物学系,江苏南京210029;南京军区疾病预防控制中心疾病预防控制所,江苏南京210002 4. 中国药科大学生命科学与技术学院,江苏南京,210009 5. 南京军区疾病预防控制中心疾病预防控制所,江苏南京,210002 |
| 基金项目： | 国家自然科学基金（81172724,30972628）; 江苏省自然科学基金面上项目（BK2010112）; 江苏省高校自然科学研究项目（09KJB330001）; 中国博士后科学基金（20090451569）; 江苏省博士后科研资助计划（0901060C） |
| 摘 要： | 目的 研究丙型肝炎病毒(hepatitis C virus,HCV)基因1b型F蛋白对HepG2细胞中Bcl-2、Bax表达的影响.方法 应用聚合酶链式反应(polymerase chain reaction,PCR)技术扩增HCV 1b型的F基因,构建pEGFP-C3-F、pEGFP-C3-C真核表达载体,分别将pEGFP-C3-F、pEGFP-C3-C、pEGFP-C3(阴性对照)瞬时转染HepG2细胞,48h后抽细胞总蛋白质,蛋白质印迹法(Western blot)检测Bcl-2、Bax表达的变化.结果 转染F基因的HepG2中Bcl-2表达水平略高于未转染的细胞,但Bax表达水平显著高于未转染的细胞.结论 HCV F蛋白具有调节原癌基因Bcl-2和抑癌基因Bax的特性,可能与肝癌的形成有关. |
| 关 键 词： | 肝炎病毒属 F蛋白 Bcl-2 Bax |
Hepatitis C virus F protein up-regulates Bcl-2 and Bax in human hepatoma HepG2 cells |
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| WEI Juan , KONG Jing , DENG Xiao-zhao , CHU Chun-li , YU Xiao-jie , YUE Ming , ZHANG Yun , XU Xiao-dong.Hepatitis C virus F protein up-regulates Bcl-2 and Bax in human hepatoma HepG2 cells[J].Chinese Journal of Disease Control and Prevention,2012,16(4):289-292. | |
| Authors: | WEI Juan KONG Jing DENG Xiao-zhao CHU Chun-li YU Xiao-jie YUE Ming ZHANG Yun XU Xiao-dong |
| Institution: | 1.1.Department of Biochemistry and Molecular Biology,School of Basic Medicine,Nanjing Medical University,Nanjing 210029,China;2.Faculty of Life Science and Chemical Engineering,Huaiyin Institute of Technology,Huaian 223300,China;3.Institute of Disease Control and Prevention,Center for Disease Control and Prevention of Nanjing Command,Nanjing 210002;4.College of Biological Science and Technology,China Pharmaceutical University,Nanjing 210009,China |
| Abstract: | Objective To study the regulatory effect of Hepatitis C Virus F protein on Bcl-2 and Bax.Methods Full length F gene of HCV was amplified by PCR,using the plasmid containing HCV 1b Full length open reading frame as template.HCV F gene-containing,plasmid pEGFP-C3-F and HCV core gene-containing pEGFP-C3-C were constructed and transiently transfected into HepG2 cells.48h posttransfection,Western blotting was used to determine the changes at translation levels of Bcl-2 and Bax genes.Results The levels of Bcl-2 expression were a little higher in pEGFP-C3-F transfected HepG2 cells than that of non-transfected cells,but significantly higher levels of Bax expression.Conclusions F protein is of regulatory properties in celluar oncogen Bcl-2 and anti-oncogen Bax,which may be implicated in the formation of hepatocelluar carcinoma. |
| Keywords: | Hepacivirus F protein Bcl-2 Bax |
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