| 新型牙本质黏结剂体外细胞毒性研究 |
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| 引用本文： | 谢超,何惠明,赵信义,李焰,张翔. 新型牙本质黏结剂体外细胞毒性研究[J]. 牙体牙髓牙周病学杂志, 2005, 15(2): 84-87 |
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| 作者姓名： | 谢超 何惠明 赵信义 李焰 张翔 |
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| 作者单位： | 第四军医大学口腔医学院,陕西,西安,710032 |
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| 摘 要： | 目的：评价一种新型牙本质黏结剂的细胞毒性，并与商品化的两种黏结剂细胞毒性进行比较。方法：采用琼脂覆盖法和四甲基偶氮唑盐(MTT)比色法，评价新型牙本质黏结剂优邦(UB)和商品化黏结剂Adper Prompt自酸蚀黏结剂(AP)与Prime＆Bond NT(PB)在人牙本质片上使用后，对L929小鼠成纤维细胞的毒性作用。结果：MTT实验中三种牙本质黏结剂细胞毒性均为1级；琼脂覆盖实验中，三种材料两种厚度牙本质片均出现脱色区和部分细胞溶解，细胞毒性评价均为1级。结论：新型牙本质黏结剂具有轻做的细胞毒性，UB对牙髓组织的影响还有待进一步研究。
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| 关 键 词： | 牙本质黏结剂 细胞毒性 琼脂覆盖法 四甲基偶氮唑盐比色法 |
| 文章编号： | 1005-2593(2005)02-0084-04 |
| 修稿时间： | 2004-08-30 |
In vitro cytotoxic study of a new dentin bonding agents |
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| Xie Chao,He Hui-ming,ZHAO Xin-yi,LI Yan,ZHANG Xiang. In vitro cytotoxic study of a new dentin bonding agents[J]. Chinese Journal of Conservative Dentistry, 2005, 15(2): 84-87 |
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| Authors: | Xie Chao He Hui-ming ZHAO Xin-yi LI Yan ZHANG Xiang |
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| Abstract: | AIM: To evaluate the cytotoxic effect of a new dentine-bonding agents. METHODS: Using agar overlay test and tetrazolium-based colorimetric assay(MTT) in vitro,the cytotoxicity of three dentine-bonding agents,U Bond(UB),Adper Prompt Self-etching Adhesive(AP),Prime & Bond NT(PB),to L929 mouse fibroblast were evaluated.The test materials were applied on the dentine discs derived from human third molars in the same way as in the clinical procedures recommented by each manufacturer. RESULTS: Cytotoxicity of the three dentine-bonding agents was 1 grade according to MTT assay.In agar overlay test,parts of decolored zones and some dissolved cells were observed under all test dentine discs. CONCLUSION: The new adhesive had slight cytotoxicity.Further study on the influence of the new dentin bonding agents towards pulp tissue must be taken. |
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| Keywords: | dentine-bonding agents cytotoxicity agar overlay test tetrazolium-based colorimetric assay |
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