脓毒血症患者血小板参数变化及其与血小板膜糖蛋白的关系

目的：探讨脓毒血症患者血小板参数变化及其与血小板膜糖蛋白的关系,以寻求精确判断血小板功能状态的办法。方法：选取2013年2月-2014年1月于本院住院治疗的脓毒症患者48例为病例组,并选取同期健康体检者32例作为对照组,应用全自动血球分析仪检测血小板参数PC、MPV、PDW,采用流式细胞仪检测血小板膜糖蛋白PAC-1、CD62P的水平。结果：与对照组比较,病例组PC显著降低,MPV、PDW均显著增大,PAC-1、CD62P均表达上调,差异均有统计学意义（P〈0.05）;Pearson相关性分析显示,MPV与PAC-1、CD62P表达水平均呈明显正相关（r=0.377、0.306,P〈0.05）,PV、PDW与PAC-1、CD62P表达水平之间无明显相关性（P〉0.05）。结论：脓毒症患者体内存在血小板明显活化,血小板参数与血小板膜糖蛋白PAC-1、CD62P共同参与血小板活化过程。

Changes of Platelet Parameters in Patients with Sepsis and Its Relationship with Platelet Membrane Glycoprotein

Objective：To investigate the changes of platelet parameters in patients with sepsis and its relationship with Platelet membrane glycoprotein, in order to estimate accurately the function of platelet. Method：48 patients with sepsis treated in our hospital from February 2013 to January 2014 were selected as sepsis group, and 32 healthy subjects were selected as control group, the platelet parameters PC, MPV, PDW were detected with automatic blood cell analyzer, and the platelet membrane glycoprotein PAC-1, CD62 P were detected with flow cytometry.Result：Compared with the control group, PC of the sepsis group was significantly decreased, MPV, PDW were significantly increased, the expression of PAC-1, CD62 P were upregulated,there were statistically significant difference between the two groups（P〈0.05）. Pearson correlation analysis showed that, there were a significant positive correlation between MPV and the expression of PAC-1, CD62P（r=0.377,0.306, P〈0.05）,and non significant correlation between PDW, PV and the expression of PAC-1, CD62P（P〈0.05）.Conclusion：Platelet was activated in patient with sepsis, and platelet parameters and platelet membrane glycoprotein PAC-1, CD62 P pay a role in the pathological process.