新疆90例贲门癌中IGF-IR、bcl-2、PCNA的表达及其意义

目的：探讨胰岛素样生长因子-I受体（IGF-IR）、细胞凋亡相关基因-2（bcl-2）及增殖细胞核抗原（PCNA）的异常表达在新疆3个不同民族贲门癌发生发展中的作用及其病理生物学意义,为此病的防治提供实验资料. 方法：应用免疫组化LSAB法检测90例贲门腺癌和21例非癌组织的IGF-IR、bcl-2、PCNA蛋白的阳性表达情况. 结果：（1）IGF-IR、bcl-2蛋白、PCNA在90例贲门腺癌中的检出率分别为84.44%（69/90）、87.78%（79/90）、97.78%（88/90）,在贲门非癌良性组织中的阳性检出率分别为47.62%（10/21）、52.38%（11/21）、0%（0/21）.IGF-IR、 bcl-2、PCNA的阳性率在癌和非癌组织间均具有统计学的差异（P〈0.01）.（2）哈萨克族（哈族）、维吾尔族（维族）、汉族各民族之间IGF-IR、bcl-2、PCNA阳性率的差异均无统计学意义（P〉0.05）.（3）IGF-IR、bcl-2、PCNA在贲门腺癌不同分化程度中阳性率的差异均无统计学意义（P〉0.05）.（4）贲门腺癌中bcl-2与IGF-IR间、bcl-2与PCNA间无相关关系（P〉0.05）.结论：（1）IGF-IR蛋白、癌基因bcl-2和PCNA的异常表达在贲门癌变过程中具有重要作用.（2）贲门癌组织中IGF-IR、bcl-2、PCNA表达在肿癌不同分化程度和不同民族间无显著差别.

Biopathological significance of the abnormal expression of IGF-IR, bcl-2, PCNA in cardiac cancer tissues in Xinjiang

Objective: To detect the abnormal expression of IGF-IR protein, bcl-2 oncogene and proliferating cell nuclear antigene (PCNA) in cardiac cancer tissues in different ethnic patients, and the molecular biology of cardiac carcinogenesis. Methods: The over expression of IGF-IR, bcl-2and PCNA on paraffin embedded tumor tissues in 90 different ethnic patients with cardinal adenocarcinoma (Kazak 29, Uygur 30, Han 31) were examined by immunohistochemistry(LSAB). Results: The positive frequency of IGF-IR, bcl-2 and PCNA in cardial cancer were 84. 44% (76/90), 87. 78% (79/90), 97. 78%(88/90) respectively. And the expression of those in non-cancer tissue were 47. 62% (10/21), 52. 38% (11/21), 0%(0/21). There were statistic significances between cancer and non-cancer (P <0. 01). However, no difference of the expression of IGF-IR, bcl-2, PCNA among different ethnic as well as varing degrees of differentiation were found (P >0. 05). No significant correlationship was found between bcl-2 and PCNA, between bcl-2 and IGF-IR (P >0. 05). Conclusion: The abnormal expression of mutant IGF-IR, bcl-2 and PCNA may be an important etiopathogenesis factor for cardial carcinogenesis. No statistic difference on the expression of IGF-IR, bcl-2 and PCNA among different ethnic was found as well as varing degrees of differentiation.