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灵芝多糖对小鼠腹腔巨噬细胞蛋白激酶A活性的影响
引用本文:李明春,梁东升,许自明,雷林生,杨淑琴,孙莉莎. 灵芝多糖对小鼠腹腔巨噬细胞蛋白激酶A活性的影响[J]. 中草药, 2000, 31(5): 353-354
作者姓名:李明春  梁东升  许自明  雷林生  杨淑琴  孙莉莎
作者单位:1. 中国人民解放军401医院药剂科,青岛,266071
2. 第一军医大学药理教研室
基金项目:国家自然科学基金资助项目!No/3 940 0 165
摘    要:为观察灵芝多糖(GLB7)体外对小鼠腹腔巨噬细胞(MΦ)蛋白激酶A(PKA)活性的影响,采用反相离子对高效液相色谱法测定MΦ中RKA活力。结果表明GLB7(40mg/L)能引起小鼠腹腔MΦ中PKA活性明显升高,10min达峰值,30min恢复到基础水平。提示灵芝多糖的免疫增强作用与其增强MΦ中PKA活性有关。

关 键 词:灵芝 灵芝多糖 巨噬细胞 蛋白激酶A 小鼠
收稿时间:1999-09-20

Effects of Ganoderma (Ganoderma lucidum) Polysaccharide on PKA Activity ofMurine Peritoneal Macrophages
Li Mingchun,Liang Dongsheng,Xu Ziming,Lei Linsheng,Yang Shuqin and Sun Lisha. Effects of Ganoderma (Ganoderma lucidum) Polysaccharide on PKA Activity ofMurine Peritoneal Macrophages[J]. Chinese Traditional and Herbal Drugs, 2000, 31(5): 353-354
Authors:Li Mingchun  Liang Dongsheng  Xu Ziming  Lei Linsheng  Yang Shuqin  Sun Lisha
Affiliation:Qingdao 266071
Abstract:To investigate the effects of Ganoderma lucidum polysaccharide (GLB 7) on protein kinase A (PKA) activity of murine peritoneal macrophages. A new ionpair RP HPLC method was used to determine the activty of PKA in cells. The results showed that GLB 7 (40 mg/L) activated the PKA of murine peritoneal macrophages, to a peak value within 10 min, and then recovered to its basic level after 30 min. This result indicated that GLB 7 may act as an immunopoteniator of PKA in murine peritoneal macrophages.
Keywords:Ganoderma lucidum (Leyee.ex Fr.) Karst Ganoderma lucidum polysaccharide macrophage protein kinase A (PKA)
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