| 胰腺癌细胞系中miRNAs的表达分析 |
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| 作者单位： | Department of Gastroenterology,Sun Yat-Sen Memorial Hospital,Sun Yat-Sen University,Guangzhou 510120,China |
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| 摘 要： |
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| 关 键 词： | miRNAs 胰腺癌 治疗 化疗 临床 |
Expression profiles of miRNAs in human pancreatic cancer cell lines |
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| Shineng Zhang,Haijun Zuo,Zhong Yu,Fengting Huang,Wa Zhong. Expression profiles of miRNAs in human pancreatic cancer cell lines[J]. The Chinese-German Journal of Clinical Oncology, 2009, 8(2): 77-80. DOI: 10.1007/s10330-008-0160-6 |
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| Authors: | Shineng Zhang Haijun Zuo Zhong Yu Fengting Huang Wa Zhong |
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| Affiliation: | Department of Gastroenterology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, China |
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| Abstract: | Objective:To analyze initially the differences of miRNAs expression profiles in human pancreatic cencer cell lines by microarray technique.Methods:A total of 743 probes were designed according to lhe known miRNAs sequences of human,mice,and rats.miRNAs microarray was manufactured and its credibility was verified.Total RNAs were extracted and miRNAs were separated from human pancreatic cancer cell lines(SW1990,Capan-2.BxPC-3,Aspc-1,and Panc 1)and immortal human pancreatic duct epithelial cell line H6C7.They were labeled with T4 RNA ligase.then were hybridized with microarray.Through array scan and analysis,miRNAs expression profiles in pancreatic cancer were obtained.The results were verified by Northern blotting and RT-PCR.Results:A totsl of 63 miRNAs related to pancreatic cancer were found to be differentially expressed in 5 pancreatic cancer cell lines.including 25 down-regulated and 38 up-regulated miRNAs.Expres- sions of mir-21 and let-7 were also confirmed.Conclusion:The results suggested that miRNAs expression profiles could befound in pancreatic cancer cells. |
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| Keywords: | pancreatic cancer miRNAs expression profiling |
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