| 强化婴幼儿奶粉中叶酸的测定 |
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| 引用本文： | 李孝宁,刘霄玲. 强化婴幼儿奶粉中叶酸的测定[J]. 营养学报, 1988, 0(4) |
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| 作者姓名： | 李孝宁 刘霄玲 |
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| 作者单位： | 上海食品工业研究所 上海(李孝宁)，上海食品工业研究所 上海(刘霄玲) |
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| 摘 要： | 强化叶酸的奶粉在0.05M K_2HPO_4缓冲液中,经均质,调pH4.5,15000g离心20分钟,其清液调pH4.0,进行CGS树脂离子交换层析,用10ml KClK_2HPO_4洗脱,调pH7.0,进行HPLC测定,采用300x3.9mm ODS反相柱,移动相0.05M K_2HPO+MeOH(91+9),流速1ml/分,紫外检测器波长285nm,灵敏度0.0050D。方法相对标准偏差为±3.3％,回收率为87％,最小检测量为0.02μg/ml。
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| 关 键 词： | 强化奶粉 叶酸 |
DETERMINATION OF FOLIC ACID IN FORTIFIED INFANT MILK POWDER BY HPLC |
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| Li Xiaoning Liu Xiaoling. DETERMINATION OF FOLIC ACID IN FORTIFIED INFANT MILK POWDER BY HPLC[J]. Acta Nutrimenta Sinica, 1988, 0(4) |
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| Authors: | Li Xiaoning Liu Xiaoling |
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| Abstract: | The fortified milk powder is dissolved in 0.05M K2HPO4 buffer solution, adjust pH to 4.5, and centrifuge for 20 minutes. The supernatant is then adjusted to pH 4.0, go through CGS resin column using 10ml KC1-K2HPO4 as eluant. The eluate is collected as testing solution and injected to HPLC. Column: ODS; mobile phase; 0.05M K2HPO4 : CH3OH (91:9); ultraviolet detector: 285nm. CV% is 3.3% and recovery 87%. |
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| Keywords: | folic acid fortified infant milk powder HPLC |
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