阿德福韦酯长期治疗对慢性乙型肝炎患者PBMCs中线粒体DNA的影响

目的探讨长期服用阿德福韦酯对CHB患者外周血单个核细胞（PBMCs）线粒体的可能损伤。方法本研究包括18例服用阿德福韦酯2年、25例服用3年和22例未服用阿德福韦酯的CHB患者；采用实时定量PCR法检测各组患者PBMCs中线粒体DNA（mtDNA）含量（mtDNA／nDMA）；采用ELISA法检测血浆丙二醛（MDA）、F2一isoprostanes含量；采用分光光度法检测血浆总抗氧化能力（TAOC）。结果2年组患者mtDNA含量为0．6±0．4copies／cell，3年组为0．8±0．5copies／cell，均显著低于对照组（1．4±1．2copies／cell，P均〈0．05）；2年组F2一iso—prostanes含量为1．5±0．8ng／ml，3年组为2．2士1．3ng／ml，显著低于对照组（3．7±2．7ng／ml，P均〈0．05）；2年组TAOC含量为3．0±1．1单位／毫升，3年组为2．3±1．4单位／毫升，显著低于对照组（4．3±1．8单位／毫升，P均〈0．05）。3组问MDA含量无统计学差异（F=2．404，P〉0．05）。结论长期应用阿德福韦酯治疗慢性乙型肝炎，对PBMCs中mtDNA水平有一定的影响，其意义还有待探讨。

The changes of mitochondrial DNA levels in peripheral blood monouclear cells in patients with chronic hepatitis b receiving long-term adefovir treatment

Objective To observe the changes of mitochondrial DNA levels in peripheral blood monouclear cells in patients with chronic hepatitis B receiving long-term adefovir treatment.Methods Patients with CHB were divided into three groups:(1) 2 years group(n=18):receiving adefovir for 2 years;(2)3 years group(n=25):adefovir for 3 years;and(3) control group(n=22):naive patients without antiviral treatment.The mtDNA of PBMCs were detected by quantitative real-time PCR.Plasma MDA and F2-isoprostanes were measured by ELISA and TAOC were detected by spectrophotometry.Results The mtDNA in PBMCs in the 2-year treatment group was 0.6±0.4 copies/cell and in the 3-year treatment group was 0.8±0.5 copies/cell,both significantly lower than in the control group(1.4±1.2copies/cell,P<0.05);the F2-isoprostanes in 2-year treatment group was 1.5±0.8 ng/ml and in 3-year treatment group was 2.2±1.3ng/ml,much lower than that in the control group(3.7±2.7 ng/ml,P<0.05);the TAOC in 2-year treatment group was 3.0±1.1 IU/ml,and in 3-year treatment group was 2.3±1.4IU/ml,significantly lower than in the control group(4.3±1.8 IU/ml,P<0.05).Conclusion The long-term administration of adefovir might injure mitochondrial DNA.