Analysis of polymorphisms and haplotypes in genes associated with vascular tone,hypertension and oxidative stress in Mexican-Mestizo women with severe preeclampsia |
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| Authors: | Ramón Mauricio Coral-Vázquez Juan Fernando Romero Arauz Samuel Canizales-Quinteros Agustín Coronel Elith Yazmin Valencia Villalvazo Jaime Hernández Rivera Belem Ramírez Regalado David Rojano Mejía Patricia Canto |
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| Affiliation: | 1. Sección de Posgrado, Escuela Superior de Medicina, Instituto Politécnico Nacional, México, D.F., Mexico;2. Subdirección de Enseñanza e Investigación, Centro Médico Nacional “20 de Noviembre,” Instituto de Seguridad y Servicios Sociales de los Trabajadores del Estado, México, D.F., Mexico;3. Hospital de Ginecología y Obstetricia 4, Luis Castelazo Ayala, Instituto Mexicano del Seguro Social, México D,F., Mexico;4. Facultad de Química, Universidad Nacional Autónoma de México, México, D.F., México, Unidad de Biología Molecular y Medicina Genómica, Instituto Nacional de Ciencias Médicas y Nutrición “Salvador Zubirán”, México, D.F., Mexico;5. División de Investigación Biomédica, Subdirección de Enseñanza e Investigación, Centro Médico Nacional 20 de Noviembre, Instituto de Seguridad y Servicios Sociales de los Trabajadores del Estado, México, D.F., Mexico;6. Doctorado en Genética Humana, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara Jalisco, Mexico;7. Hospital Regional Adolfo López Mateos, Instituto de Seguridad y Servicios Sociales de los Trabajadores del Estado, México, D.F., Mexico;8. Unidad de Medicina Física y Rehabilitación, Región Centro, UMAE Lomas Verdes, Instituto Mexicano del Seguro Social, México, D.F., Mexico |
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| Abstract: | ObjectiveSeveral studies have reported the association of genes related to vascular tone, hypertension, oxidative stress and preeclampsia. We investigated the possible association among three polymorphisms in eNOS (as well their haplotypes): one of MTHFR, one of GSTP1 and one of AGT, with severe preeclampsia in Mexican-Mestizo women.MethodsTwo hundred thirty women with severe preeclampsia and 350 control subjects were genotyped; for rs2070744 and rs1799983 of eNOS, rs1801133 of MTHFR, rs1695 of GSTP1 and rs699 of AGT we used real-time PCR allelic discrimination and for VNTR of eNOS, PCR. Allele frequency differences were assessed by χ2. Logistic regression was used to test for associations and for haplotype frequencies using Haploview 4.2.ResultsGenotypic and allelic distribution of the polymorphisms was similar between cases and controls; likewise, haplotype frequencies of the three polymorphisms of eNOS did not differ significantly.ConclusionsTo our knowledge, this is the first time that these polymorphisms have been analyzed together and exclusively in women with severe preeclampsia. However, we did not find an association between polymorphisms of eNOS, MTHFR, GSTP1 and AGT with severe preeclampsia in our population. Additionally, we observed differences in the distribution of the alleles and genotypes of these polymorphisms in our population in comparison to those described in other ethnic groups. |
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