Telomerase as a potential marker for inflammation and cancer detection in bronchial washing: A prospective study |
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| Authors: | Najib Nassani Georges Khayat Issam Raad Ying Jiang Nada Alaaeddine George Hilal |
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| Affiliation: | 1. Cancer and Metabolism Research Laboratory, Saint-Joseph University, Faculty of Medicine, Beirut, Lebanon;2. Pulmonary and Critical Care Division, Hotel-Dieu de France Hospital, Saint-Joseph University, Faculty of Medicine, Beirut, Lebanon;3. Infectious Diseases, Infection Control and Employee Health, the University of Texas, M.D. Anderson Cancer Center, Houston, TX, USA;1. Department of Cardiology, The First Affiliated Hospital of Harbin Medical University, Harbin, China;2. Department of Emergency, The First Affiliated Hospital of Harbin Medical University, Harbin, China;1. Department of Gastroenterology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China;2. Department of Radiation Oncology, The First Affiliated Hospital of Wannan Medical College, Wuhu 241001, China;3. Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China;1. Department of Cardiothoracic Surgery, Affiliated People''s Hospital of Jiangsu University, Zhenjiang 212002, China;2. Department of Cardiovascular Medicine, Cardiovascular Clinical College of Tianjin Medical University & TEDA International Cardiovascular Hospital, Tianjin 300457, China;3. Department of Cardiology, Wuxi People''s Hospital Affiliated to Nanjing Medical University, Wuxi 214002, China;1. Dept. of Medical Sciences, Cardiology, Uppsala University, 75237 Uppsala, Sweden;2. Dept. of Medical Sciences, Clinical Chemistry, Uppsala University, 75237 Uppsala, Sweden;3. Uppsala Clinical Research Center, Uppsala University, 75237 Uppsala, Sweden;4. Dept. of Medicine, Division of Cardiology, University of Alberta, Edmonton, AB T6G2H7, Canada;5. Duke Translational Medicine Institute, Duke University School of Medicine, 2301 Erwin Road, Durham, NC 27710, USA;6. Thoraxcenter, Dept. of Cardiology, Erasmus Medical Center, ''s Grafendijkwal 230, 3000 CA Rotterdam, The Netherlands |
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| Abstract: | ![]()
ObjectivesThe diagnosis of lung cancer remains difficult especially in peripheral tumors, given the absence of relevant markers and of sensitive imaging techniques. Telomerase is a ribonucleotide enzyme responsible for the immortalization of cancerous cells and seems to increase in bronchial aspirates of lung cancer patients. The purpose of our study is to further investigate the value of telomerase measurement in bronchial aspirates as a diagnostic tool for lung cancer.Design and methodsRandom 82 bronchial aspirates were obtained from patients undergoing bronchoscopy to diagnose any lung illness including inflammation and cancer. Cytology examination, quantification of proteins by Bradford method, and telomerase activity measurement by quantitative Real-time PCR were performed. Out of 82 specimens, 11 were excluded because of hemolysis, absence of elements or lack of final diagnosis. ROC curve analysis was done.ResultsA significant difference in telomerase activity average was noted between normal patients and those with inflammation and cancer. Discriminatory capacity of telomerase activity was: for cancer vs. non cancer, AUC = 0.74 (95% CI: 0.62–0.84), sensitivity = 78%, specificity = 72%, Negative Predictive Value = 87%, at cut-off > 0.46 atmol/mg protein/20 min; for cancer vs. normal, AUC = 0.87 (95% CI: 0.72–0.96), se = 78%, sp = 92%, NPV = 71%, at cut-off > 0.46; for cancer vs. inflammation, AUC = 0.69 (95% CI: 0.55–0.80), se = 74%, sp = 70%, NPV = 79%, at cut-off > 1.03, and for inflammation vs. normal, AUC = 0.76 (95% CI: 0.62–0.88), se = 79%, sp = 77%, NPV = 59%, at cut-off > 0.ConclusionTelomerase activity in bronchial aspirates is a promising diagnostic marker for lung cancer and inflammation detection. |
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