Characterization of IL-2 responsive synovial T lymphocytes in rheumatoid arthritis |
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| Authors: | W. A. Ofosu-Appiah R. J. Warrington J. A. Wilkins |
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| Affiliation: | (1) Rheumatic Disease Unit Research Laboratory, RRO14, University of Manitoba, 800 Sherbrook Street, R3A 1M4 Winnipeg, Manitoba, Canada;(2) Department of Immunology, University of Manitoba, R3A 1M4 Winnipeg, Manitoba, Canada;(3) Department of Medicine, University of Manitoba, R3A 1M4 Winnipeg, Manitoba, Canada;(4) Department of Medical Microbiology, University of Manitoba, R3A 1M4 Winnipeg, Manitoba, Canada |
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| Abstract: | ![]()
Summary Lymphocytes from peripheral blood (PBL) and synovial fluid (SFL) were obtained from patients with rheumatoid arthritis (RA) and cloned under limiting-dilution conditions without prior activation but in the presence of exogenous interleukin (IL)-2. The precursor frequencies of such in vivo activated IL-2-responsive cells were higher in RA SFL (1/83) than in RA PBL (1/201) or normal PBL (1/377). These HLA-Dr/Ia-positive clones expressed T-cell markers CD3 and T101 and were either CD4- or CD8-positive but lacked NK markers CD11, CD16, and HNK-1. All such clones were cytotoxic for NK-sensitive K562 targets and NK-insensitive Raji cell targets. These cells, which most closely resemble non-major histocompatibility complex (MHC) restricted cytotoxic T (CTL) cells, are present with increased frequency in RA synovial fluids. |
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| Keywords: | Synovial T cells Non-MHC restricted CTL cells Rheumatoid arthritis |
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