|
|||||
|
|
Hypoxia-Inducible Factor 1-Alpha Release After Intracoronary Versus Intramyocardial Stem Cell Therapy in Myocardial Infarction |
|
| Authors: | Mariann Gyöngyösi Rayyan Hemetsberger Aniko Posa Silvia Charwat Noemi Pavo Örs Petnehazy Zsolt Petrasi Imre J. Pavo Hani Hemetsberger Imre Benedek Teodora Benedek Istvan Benedek Jr Istvan Kovacs Christoph Kaun Gerald Maurer |
| Affiliation: | 1. Department of Cardiology, Medical University of Vienna, W?hringer Gürtel 18-20, 1090, Vienna, Austria 2. Institute of Diagnostic Imaging and Radiation Oncology, University of Kaposvar, 7400, Kaposvár, Guba S. 40, Hungary 3. University of Targu Mures, Str. Gh. Marinescu nr. 50, Targu-Mure?, Romania |
| Abstract: | We have investigated the effect of stem cell delivery on the release of hypoxia-inducible factor 1 alpha (HIF-1α) in peripheral circulation and myocardium in experimental myocardial ischemia. Closed-chest, reperfused myocardial infarction (MI) was created in domestic pigs. Porcine mesenchymal stem cells (MSCs) were cultured and delivered (9.8?±?1.2?×?106) either percutaneously NOGA-guided transendocardially (Group IM) or intracoronary (Group IC) 22?±?4 days post-MI. Pigs without MSC delivery served as sham control (Group S). Plasma HIF-1α was measured at baseline, immediately post- and at follow-up (FUP; 2 h or 24 h) post-MSC delivery by ELISA kit. Myocardial HIF-1α expression of infarcted, normal myocardium, or border zone was determined by Western blot. Plasma level of HIF-1α increased immediately post-MI (from 278?±?127 to 631?±?375 pg/ml, p?p?p? |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |