Method for the rapid determination of norepinephrine,dopamine, and serotonin in the same brain region

A method is presented for the fluorometric analysis of norepinephrine, dopamine and serotonin. This procedure is a combination of an unpublished catecholamine assay developed by Hogans and of the o-phthaldialdehyde serotonin reaction reported by Maickel and Miller [9]. This procedure should greatly facilitate the correlation of neurotransmitter levels in brain regions with changes in behavior produced by experimental manipulations.