| 兔腰椎融合术中BMP和bFGF对骨髓间充质干细胞的作用 |
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| 引用本文： | 臧业峰,刘亚,邱玉金. 兔腰椎融合术中BMP和bFGF对骨髓间充质干细胞的作用[J]. 潍坊医学院学报, 2008, 30(6) |
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| 作者姓名： | 臧业峰 刘亚 邱玉金 |
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| 作者单位： | 潍坊医学院外科学教研室,山东,潍坊,261053 |
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| 摘 要： | 目的评价骨形态发生蛋白(BMP)和碱性成纤维细胞生长因子(bFGF)诱导的兔骨髓间充质干细胞(MSC)复合多孔生物陶瓷对提高腰椎融合率的作用。方法将32只家兔随机分为4组(n=8),用下列移植物分别进行腰椎后外侧路融合:Ⅰ组单独应用MSC;Ⅱ组以bFGF作用MSC;Ⅲ组用BMP-2作用MSC;Ⅳ组用BMP-2和bFGF共同作用MSC。实验动物术后6周处死,利用放射学、手工生物力学和组织学方法观察和分析椎体融合的情况。结果影像学和手工力学检测,Ⅳ组椎体融合情况要比其它3组好得多;组织学检测示,在Ⅳ组中,可以看到在融合区周围出现大量的新生成的骨小梁。Ⅱ,Ⅲ组在融合区新生骨小梁较Ⅳ组少,而是新生骨小梁与纤维结缔组织混杂出现;Ⅰ组新生骨小梁显著减少,生物陶瓷微孔内多为纤维结缔组织。结论经BMP和bFGF体外培养的MSC可取得比单独应用MSC更好的融合效果。
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| 关 键 词： | 骨形态发生蛋白 碱性成纤维细胞生长因子 骨髓间充质干细胞 脊柱融合 |
The Effect of Bone Morphogenetic Protein and Basic Fibroblast Growth Factor on Cultured Mesenchymal Stem Cells for Rabbit Lumbar Spinal Fusion |
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| ZANG Ye-feng,LIU Ya,QIU Yu-jin. The Effect of Bone Morphogenetic Protein and Basic Fibroblast Growth Factor on Cultured Mesenchymal Stem Cells for Rabbit Lumbar Spinal Fusion[J]. Journal of Weifang Medical College, 2008, 30(6) |
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| Authors: | ZANG Ye-feng LIU Ya QIU Yu-jin |
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| Affiliation: | ZANG Ye-feng; LIU Ya; QIU Yu-jin (Department of Surgery; Weifang Medical College; Weifang 261053); |
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| Abstract: | Objectives To determine the ability of basic FGF to enhance the efficacy of bone marrow-derived mesenchymal stem cells in lumbar arthrodesis.Methods Thirty-two adult rabbits underwent singlelevel bilateral posterolateral intertransverse process fusions at L_4-L_5.The animals were divided into 4 groups,each according to the material implanted:group,mesenchymal stem cells(n=8); groupⅡ,mesenchymal stem cells with recombinant human bone morphogenetic protein(rhBMP)-2(MSC-BMP;n=8);groupⅢ,mesenchymal stem cells w... |
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| Keywords: | Bone morphogenetic protein Basic fibroblast growth factor Mesenchymal stem cell Spinal fusion |
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